Properties of an xeroderma pigmentosum revertant cell line expressing endonuclease V
| Autor: | Earl E. Henderson, Anthony P. Green, Jon K. deRiel |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Xeroderma pigmentosum
Transcription Genetic Molecular Sequence Data Biophysics Pyrimidine dimer Biology medicine.disease_cause Biochemistry Polymerase Chain Reaction Cyclobutane Cell Line chemistry.chemical_compound Endonuclease Deoxyribonuclease (Pyrimidine Dimer) Shuttle vector medicine Humans Molecular Biology Cell Line Transformed Mutation Xeroderma Pigmentosum Endodeoxyribonucleases Base Sequence Mutagenesis Methylnitrosourea Cell Biology DNA medicine.disease Virology Molecular biology chemistry Genes Oligodeoxyribonucleotides biology.protein RNA |
| Zdroj: | Biochemical and biophysical research communications. 190(1) |
| ISSN: | 0006-291X |
| Popis: | We have developed a set of cell lines to help distinguish the sequelae of specific lesions in DNA after UV irradiation. Irradiation results in two primary lesions: cyclobutane dimers and pyrimidine-pyrimidone (6-4) photoproducts. The contributions of each to mutation are considered utilizing a spectrum of cell lines with increasing abilities to repair these lesions. In particular, we focus on a revertant of the XP12Ro(M1) cell line from a patient with Xeroderma pigmentosum, XP129, which is capable of repairing (6-4) photoproducts but not cyclobutane dimers. We have successfully introduced the denV gene into these cells which confers the ability to repair cyclobutane dimers. By comparing the results of a shuttle vector mutation experiment with the vector pZ189, we can correlate specific mutations to specific lesions. |
| Databáze: | OpenAIRE |
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