Composition-function analysis of HDL subpopulations: influence of lipid composition on particle functionality
Autor: | Zsuzsanna Kuklenyik, Katrin Niisuke, Katalin V. Horvath, Michael S. Gardner, Bela F. Asztalos, Christopher A. Toth |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Adult Male Phospholipid Coronary Disease QD415-436 030204 cardiovascular system & hematology Biochemistry 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine Endocrinology High-density lipoprotein lipid ratios Humans Particle Size phospholipids Research Articles pre-beta Aged Triglyceride Cholesterol Cell Biology Lipid Droplets Middle Aged 030104 developmental biology chemistry high density lipoprotein Cholesteryl ester Biophysics Particle Composition (visual arts) lipids (amino acids peptides and proteins) Female Particle size Lipoproteins HDL |
Zdroj: | J Lipid Res Journal of Lipid Research, Vol 61, Iss 3, Pp 306-315 (2020) |
ISSN: | 1539-7262 |
Popis: | The composition-function relationship of HDL particles and its effects on the mechanisms driving coronary heart disease (CHD) is poorly understood. We tested the hypothesis that the functionality of HDL particles is significantly influenced by their lipid composition. Using a novel 3D-separation method, we isolated five different-sized HDL subpopulations from CHD patients who had low preβ-1 functionality (low-F) (ABCA1-dependent cholesterol-efflux normalized for preβ-1 concentration) and controls who had either low-F or high preβ-1 functionality (high-F). Molecular numbers of apoA-I, apoA-II, and eight major lipid classes were determined in each subpopulation by LC-MS. The average number of lipid molecules decreased from 422 in the large spherical α-1 particles to 57 in the small discoid preβ-1 particles. With decreasing particle size, the relative concentration of free cholesterol (FC) decreased in α-mobility but not in preβ-1 particles. Preβ-1 particles contained more lipids than predicted; 30% of which were neutral lipids (cholesteryl ester and triglyceride), indicating that these particles were mainly remodeled from larger particles not newly synthesized. There were significant correlations between HDL-particle functionality and the concentrations of several lipids. Unexpectedly, the phospholipid:FC ratio was significantly correlated with large-HDL-particle functionality but not with preβ-1 functionality. There was significant positive correlation between particle functionality and total lipids in high-F controls, indicating that the lipid-binding capacity of apoA-I plays a major role in the cholesterol efflux capacity of HDL particles. Functionality and lipid composition of HDL particles are significantly correlated and probably both are influenced by the lipid-binding capacity of apoA-I. |
Databáze: | OpenAIRE |
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