Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli
| Autor: | Floriana Rotondo, Xavier Remesar, María del Mar Romero, Ana Cecilia Ho-Palma, Marià Alemany, José Antonio Fernández-López |
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| Přispěvatelé: | Universitat de Barcelona |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Glycerol Male medicine.medical_specialty Histology Rats as laboratory animals Adipose tissue 030209 endocrinology & metabolism White adipose tissue Carbohydrate metabolism 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Adipocyte Internal medicine medicine Adipocytes Animals Carbon Radioisotopes Lactic Acid Rats Wistar Rates (Animals de laboratori) Cells Cultured Epididymis Chemistry Lipogenesis Adipose tissues Cell Biology Metabolism Metabolisme dels lípids Rats Teixit adipós 030104 developmental biology Endocrinology Lipid metabolism Glucose Anaerobic glycolysis Glucosa Research Paper |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname Dipòsit Digital de la UB Universidad de Barcelona |
| Popis: | White adipose tissue can metabolize large amounts of glucose to glycerol and lactate. We quantitatively traced glucose label to lactate, glycerol and fats in primary cultures of mature rat epididymal adipocytes. Cells were incubated with 7/14 mM (14)C-glucose for 24/48 h. Medium metabolites and the label in them and in cells' components were measured. Gene expression analysis was done using parallel incubations. Glucose concentration did not affect lactate efflux and most parameters. Glycerol efflux increased after 24 h, coinciding with arrested lipogenesis. Steady production of lactate was maintained in parallel to glycerogenesis. Changes in adipocyte metabolism were paralleled by gene expression. Glucose use for lipogenesis was minimal, and stopped (24 h-onwards) when glycerol efflux increased because of triacylglycerol turnover. Lactate steady efflux showed that anaerobic glycolysis was the main adipocyte source of energy. We can assume that adipose tissue may play a quantitatively significant effect on glycaemia, returning 3C fragments thus minimizing lipogenesis. |
| Databáze: | OpenAIRE |
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