Potential use of IgG2-ELISA in the diagnosis of chronic elephantiasis and IgG4-ELISA in the follow-up of microfilaraemic patients infected with Brugia malayi
| Autor: | R. Mehdi, A.W. Omar, N. Rahmah, B. Sinniah, A.K. Anuar, R. Karim |
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| Rok vydání: | 1994 |
| Předmět: |
Helminth infections
Biophysics Antibodies Helminth Enzyme-Linked Immunosorbent Assay Elephantiasis Optical density Biochemistry Brugia malayi Filariasis Elephantiasis Filarial parasitic diseases Medicine Animals Humans Molecular Biology Helminthic infections biology business.industry Cell Biology medicine.disease biology.organism_classification Immunoglobulin G Immunology Chronic Disease business Follow-Up Studies |
| Zdroj: | Biochemical and biophysical research communications. 205(1) |
| ISSN: | 0006-291X |
| Popis: | Sera from fifty subjects with different presentations of Brugian filariasis and from common soil-transmitted helminth infections were tested for specific anti-filarial IgG and its subclasses. Anti-filarial IgG, IgG1 and IgG3 showed crossreactivities with soil-transmitted helminthic infections and no significant differences in optical densities among the various groups of filarial patients. In comparison with other groups of subjects, IgG4-ELISA of sera from microfilaraemic patients and some previously microfilaraemic patients showed a significant increase in optical density readings, while IgG2-ELISA showed elevated optical density readings in sera of patients with chronic elephantiasis. Therefore IgG2-ELISA is potentially useful in the diagnosis of brugian chronic elephantiasis while IgG4-ELISA may be beneficial for follow-up diagnosis of treated microfilaraemic patients. |
| Databáze: | OpenAIRE |
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