Development of osteopromotive poly (octamethylene citrate glycerophosphate) for enhanced bone regeneration
| Autor: | Di Lu, Jian Yang, Sarah K. Chomos, Yun He, Chuying Ma, Qiyao Li, John William Tierney, Denghui Xie, Limei Li, Li Gui, Yitao Zhao, Dingying Shan, Cheng Dong, Lin Sun |
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| Rok vydání: | 2019 |
| Předmět: |
Bone Regeneration
Polymers 0206 medical engineering Composite number Biomedical Engineering chemistry.chemical_element Biocompatible Materials 02 engineering and technology Calcium Biochemistry Article Bone and Bones Osseointegration Biomaterials Tissue engineering Osteogenesis Tensile Strength Ultimate tensile strength Cell Adhesion Animals Humans Citrates Microparticle Bone regeneration Molecular Biology chemistry.chemical_classification Tissue Engineering Tissue Scaffolds Cell Differentiation Mesenchymal Stem Cells General Medicine Polymer 021001 nanoscience & nanotechnology 020601 biomedical engineering Durapatite chemistry Glycerophosphates Models Animal Hip Prosthesis Rabbits 0210 nano-technology Biotechnology Biomedical engineering |
| Zdroj: | Acta Biomater |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2019.03.050 |
| Popis: | The design and development of bioactive materials that are inherently conducive for osteointegration and bone regeneration with tunable mechanical properties and degradation remains a challenge. Herein, we report the development of a new class of citrate-based materials with glycerophosphate salts, β-glycerophosphate disodium (β-GP-Na) and glycerophosphate calcium (GP-Ca), incorporated through a simple and convenient one-pot condensation reaction, which might address the above challenge in the search of suitable orthopedic biomaterials. Tensile strength of the resultant poly (octamethylene citrate glycerophosphate), POC-βGP-Na and POC-GP-Ca, was as high as 28.2 ± 2.44 MPa and 22.76 ± 1.06 MPa, respectively. The initial modulus ranged from 5.28 ± 0.56 MPa to 256.44 ± 22.88 MPa. The mechanical properties and degradation rate of POC-GP could be controlled by varying the type of salts, and the feeding ratio of salts introduced. Particularly, POC-GP-Ca demonstrated better cytocompatibility and the corresponding composite POC-GP-Ca/hydroxyapatite (HA) also elicited improved osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro, as compared to POC-βGP-Na/HA and POC/HA. The superior in-vivo performance of POC-GP-Ca/HA microparticle scaffolds in promoting bone regeneration over POC-βGP-Na/HA and POC/HA was further confirmed in a rabbit femoral condyle defect model. Taken together, the tunability of mechanical properties and degradation rates, together with the osteopromotive nature of POC-GP polymers make these materials, especially POC-GP-Ca well suited for bone tissue engineering applications. Statement of Significance The design and development of bioactive materials that are inherently conducive for osteointegration and bone regeneration with tunable mechanical properties and degradation remains a challenge. Herein, we report the development of a new class of citrate-based materials with glycerophosphate salts, β-glycerophosphate disodium (β-GPNa) and glycerophosphate calcium (GPCa), incorporated through a simple and convenient one-pot condensation reaction. The resultant POC-GP polymers showed significantly improved mechanical property and tunable degradation rate. Within the formulation investigated, POC-GPCa/HA composite further demonstrated better bioactivity in favoring osteogenic differentiation of hMSCs in vitro and promoted bone regeneration in rabbit femoral condyle defects. The development of POC-GP expands the repertoire of the well-recognized citrate-based biomaterials to meet the ever-increasing needs for functional biomaterials in tissue engineering and other biomedical applications. |
| Databáze: | OpenAIRE |
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