Immunization with WT1-derived peptides by tattooing inhibits the growth of TRAMP-C2 prostate tumor in mice
| Autor: | Petr Hainz, K. Babiarova, Jitka Krystofova, Kamila Zurkova, Eva Brabcova, Sarka Nemeckova, L. Kutinová, Jan Musil |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Cancer Research Injections Intradermal medicine.drug_class CpG Oligodeoxynucleotide medicine.medical_treatment Immunology Antineoplastic Agents urologic and male genital diseases Monoclonal antibody Decitabine Cancer Vaccines Mice Immune system Adjuvants Immunologic Transforming Growth Factor beta Cell Line Tumor medicine Immunology and Allergy Animals WT1 Proteins Pharmacology Mice Inbred BALB C biology Tattooing business.industry Antibodies Monoclonal Prostatic Neoplasms Poly I-C Cell culture Vaccines Subunit Peptide vaccine Cancer research biology.protein Azacitidine Antibody business Adjuvant Transforming growth factor |
| Zdroj: | Journal of immunotherapy (Hagerstown, Md. : 1997). 35(6) |
| ISSN: | 1537-4513 |
| Popis: | The expression of the transcription factor encoded by the Wilms tumor gene 1 (WT1) is associated with a variety of human cancers. WT1 protein has been reported to serve as a target antigen for tumor-specific immune responses. We observed that the immunization of mice with peptide vaccines derived from WT1 in a mixture with the CpG adjuvant (ODN 1826) by tattoo administration was superior to subcutaneous delivery of the peptides in combination with CpG formulated with the mineral oil adjuvant or a DNA vaccine or a recombinant vaccinia virus vaccine expressing the truncated WT1 protein. Tattooing with the WT1122-140 and WT1126-134 peptide elicited the response of WT1-specific interferon-γ-producing T cells. Peptide vaccine administered with a tattoo device had an antitumor effect on the growth of the prostate tumor cell line TRAMP-C2, provided that the transforming growth factor-β produced by tumor cells was neutralized by anti-TGFβ monoclonal antibody. The treatment of the tumor-bearing mice with 5-azadeoxycytidine or poly IC did not work in synergy with the peptide vaccine. |
| Databáze: | OpenAIRE |
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