Humoral autoimmune response to ribosomal P proteins in chronic Chagas heart disease
| Autor: | M.H.V. Van Regenmortel, Gabriela Levitus, M. Hontebeyrie-Joskowicz, Mariano J. Levin |
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| Rok vydání: | 1991 |
| Předmět: |
Chagas Cardiomyopathy
Ribosomal Proteins Immunology Protozoan Proteins Antibodies Protozoan medicine.disease_cause Ribosome Epitope Subclass law.invention law parasitic diseases medicine Humans Lupus Erythematosus Systemic Immunology and Allergy Trypanosoma cruzi Autoantibodies biology biology.organism_classification Isotype Virology Molecular mimicry Immunoglobulin G Antibody Formation biology.protein Recombinant DNA Antibody Research Article |
| Zdroj: | Clinical and Experimental Immunology. 85:413-417 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1111/j.1365-2249.1991.tb05741.x |
| Popis: | SUMMARY The C terminal region of a Trypanosoma cruzi ribosomal P protein, encoded by the λgtll JL5 recombinant, defined a major antigenic determinant in chronic Chagas heart disease. Immunopurified anti-JL5 antibodies were tested for anti-human ribosome reactivity by immunoblotting. They recognized the parasite ribosomal P proteins and clearly reacted with the corresponding human P proteins. The peptide R-13, that comprises the 13 C terminal residues of the JL5 recombinant and defines the specificity shared between chronic Chagas heart disease anti-JL5 antibodies and the systemic lupus erythematosus (SLE) anti-P antibodies, was used to study the specificity and the IgG subclass distribution of the anti-R-13 response by ELISA. The R-13 autoepitope is recognized mainly by sera from chagasic patients, but not by sera from malaria patients. Moreover, there was a significant correlation between anti-R-13 antibody levels and anti-T. cruzi antibody titres. The anti-R-13 response was mainly restricted to the IgG1 heavy chain isotype and correlated with the anti-T. cruzi isotype distribution. |
| Databáze: | OpenAIRE |
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