Two-pore channel 1 interacts with citron kinase, regulating completion of cytokinesis
| Autor: | Mark Speck, Clay Wakano, Alexander J. Stokes, Jaime S Horton |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
autophagy
tumor Myosin light-chain kinase Biophysics cytokinesis Protein Serine-Threonine Kinases Biology multinucleation Biochemistry Rho citron kinase Lysosome medicine Humans cancer Cells Cultured polyploidy Ion channel Cell Proliferation HEK 293 cells Intracellular Signaling Peptides and Proteins 2-pore channel Cell cycle Research Papers Cell biology abscission HEK293 Cells Two-pore channel medicine.anatomical_structure lysosome Phosphorylation cell cycle Calcium Channels TPC Cytokinesis MLC |
| Zdroj: | Channels |
| ISSN: | 1933-6969 1933-6950 |
| DOI: | 10.4161/19336950.2014.978676 |
| Popis: | Two-pore channels (TPC1, 2, and 3) are recently identified endolysosmal ion channels, but remain poorly characterized. In this study, we show for the first time a role for TPC1 in cytokinesis, the final step in cell division. HEK 293 T-REx cells inducibly overexpressing TPC1 demonstrated a lack of proliferation accompanied by multinucleation and an increase in G2/M cycling cells. Increased TPC1 was associated with a concomitant accumulation of active RhoGTP and a decrease in phosphorylated myosin light chain (MLC). Finally, we demonstrated a novel interaction between TPC1 and citron kinase (CIT). These results identify TPC1 as a central component of cytokinetic control, specifically during abscission, and introduce a means by which the endolysosomal system may play an active role in this process. |
| Databáze: | OpenAIRE |
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