Backscattered electron imaging of high pressure frozen soybean root nodules visualizes formation of symbiosome membranes
| Autor: | Nayeema Bulbul, Kimie Atsuzawa, Farhana Sharmin, Yasuko Kaneko |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Root nodule
Scanning electron microscope 02 engineering and technology 01 natural sciences Structural Biology 0103 physical sciences Native state Freeze Fracturing Radiology Nuclear Medicine and imaging Plastids Instrumentation 010302 applied physics Cell Nucleus Ice crystals Chemistry Vesicle Cell Membrane 021001 nanoscience & nanotechnology Mitochondria Membrane Symbiosome Biophysics Microscopy Electron Scanning Sublimation (phase transition) Soybeans 0210 nano-technology Root Nodules Plant |
| Zdroj: | Microscopy (Oxford, England). 68(2) |
| ISSN: | 2050-5701 |
| Popis: | High-pressure frozen soybean root nodules were fractured and backscattered electron images were obtained from uncoated samples in a low vacuum scanning electron microscope equipped with a cryo-transfer system. Structures of infected cells were well preserved: numerous symbiosomes, as well as nuclei, plastids and mitochondria were observed without ice crystal damage. After appropriate sublimation of water, bacteria included in symbiosomes were visualized. Membrane accumulation near nuclei, and vesicles and tubular membranes, which possibly contribute to symbiosome membrane formation, could be observed in a near native state. The method promises to be widely applicable to visualize interaction between membranes in various biological systems. |
| Databáze: | OpenAIRE |
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