Hypoglycemic effect of silychristin A from Silybum marianum fruit via protecting pancreatic islet β cells from oxidative damage and inhibiting α-glucosidase activity in vitro and in rats with type 1 diabetes
| Autor: | Ningbo Qin, Zhan-Lin Li, Xu Hu, Ming Gao, Sheng-Ge Li, Dahong Li, Fanxing Xu, Jian Wang, Hui-Ming Hua |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Medicine (miscellaneous) 030209 endocrinology & metabolism Oxidative phosphorylation Silybum marianum 03 medical and health sciences 0302 clinical medicine Western blot Internal medicine medicine TX341-641 chemistry.chemical_classification geography Reactive oxygen species Nutrition and Dietetics geography.geographical_feature_category biology medicine.diagnostic_test Streptozotocin Nutrition. Foods and food supply Pancreatic islet β cells Diabetes biology.organism_classification Islet In vitro 030104 developmental biology Endocrinology chemistry α-Glucosidase Apoptosis Silychristin A Food Science medicine.drug |
| Zdroj: | Journal of Functional Foods, Vol 38, Iss, Pp 168-179 (2017) |
| ISSN: | 1756-4646 |
| Popis: | In the present study, the hypoglycemic effect of silychristin A on streptozotocin (STZ)-induced type 1 diabetes in rats was evaluated and the underlying pharmacological mechanism was further elucidated in vitro. Silychristin A significantly lowered the glucose level, increased insulin secretion, and improved the structure of β cells in STZ-induced rats. Accordantly, reactive oxygen species (ROS) production induced by STZ or high concentration of glucose (HG) was effectively suppressed by silychristin A in pancreatic INS-1 β cells evaluated by flow cytometry. Western blot analysis demonstrated that silychristin A markedly suppressed STZ or HG induced cell apoptosis. In addition, in vitro inhibitory effect of silychristin A on α-glucosidase and molecular docking analysis revealed that silychristin A could reduce the digestion rate of carbohydrates. These findings indicate that silychristin A may be useful as a potential functional food ingredient against oxidative stress-mediated destruction of pancreatic islet β cells and inhibition of α-glucosidase activity. |
| Databáze: | OpenAIRE |
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