Doxazosin, an alpha1-adrenoceptor antagonist, inhibits serotonin-induced shape change in human platelets
| Autor: | Dimitri P. Mikhailidis, IA Jagroop |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Blood Platelets Male medicine.medical_specialty Serotonin Adrenergic receptor urologic and male genital diseases Nifedipine Internal medicine Internal Medicine medicine Doxazosin Humans Platelet skin and connective tissue diseases Adrenergic alpha-Antagonists Whole blood Cell Size Naftopidil business.industry Antagonist Middle Aged Platelet Activation Endocrinology Hypertension Female sense organs business medicine.drug |
| Zdroj: | Journal of human hypertension. 15(3) |
| ISSN: | 0950-9240 |
| Popis: | Patients with peripheral vascular disease or diabetes mellitus tend to have elevated circulating levels of naturally occurring platelet agonists like serotonin (5 hydroxytryptamine; 5-HT). This bioamine can induce platelet shape change (PSC) an early phase of platelet activation, which is essentially aspirin resistant. In addition, 5-HT exerts other harmful effects (eg stimulating vascular smooth muscle proliferation and inducing vasoconstriction in atheromatous coronary vessels). The aim of this study was to determine whether doxazosin inhibits 5-HT-induced PSC. Doxazosin is a long acting alpha(1)-adrenoceptor antagonist, used in the treatment of essential hypertension and/or benign prostatic hyperplasia (BPH). Platelet rich plasma (PRP) was prepared from healthy volunteers (n = 8; five males and three females with a median age of 32 years, range: 26-57). Agonists (5-HT, 0.06-0.5; ADP, 0.1-0.2 micromol/l or U46619, a TXA(2)analogue, 0.025-0.05 micromol/l) were added to PRP and aliquots were removed at specific time points for median platelet volume (MPV) measurement (using a high-resolution channelyser). The MPV was used as an indicator of PSC. PRP was also incubated with doxazosin (final concentration: 0.33 microM, a concentration similar to therapeutic plasma levels) prior to the addition of each of the above-mentioned agonists. Doxazosin significantly inhibited (P = 0.007 and P = 0.008, at 30 sec and 60 sec, respectively) the 5-HT-induced increase in MPV. Doxazosin did not significantly inhibit ADP- or U46619-induced PSC. The inhibitory effect of doxazosin seems to be specific to platelet 5-HT(2) receptors, since there was no effect on ADP- or U46619-induced PSC. This inhibition of platelet activation may be an additional, clinically relevant, advantage. Future in vivo studies should consider assessing the effect of doxazosin on 5-HT-induced platelet activation. |
| Databáze: | OpenAIRE |
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