Recombinant Immunoblot Assays for Hepatitis C in Human Immunodeficiency Virus Type 1-Infected US Navy Personnel
| Autor: | Peter L. Perine, Joe P. Bryan, Joseph L. Malone, Jaqueline Sheffield, Tzu-Cheg Kao, Philip Macarthy, Maria H. Sjogren, Edith S. Smith, Kenneth F. Wagner |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Hepatitis Viral Human Hepatitis C virus Immunoblotting Enzyme-Linked Immunosorbent Assay Hepacivirus medicine.disease_cause Sex Factors medicine Humans Immunology and Allergy Hepatitis Antibodies Syphilis Naval Medicine Hepatitis Analysis of Variance Hepatitis B Surface Antigens AIDS-Related Opportunistic Infections biology business.industry Hepatitis A Alanine Transaminase Hepatitis C Hepatitis B medicine.disease Virology United States Military Personnel Infectious Diseases HIV-1 Coinfection biology.protein Regression Analysis Female Antibody Viral hepatitis business |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/167.3.715 |
| Popis: | The prevalence of hepatitis A, B, C, and D viruses was studied in 467 military personnel with human immunodeficiency virus type 1 (HIV-1) infection. Antibody to hepatitis C virus (anti-HCV) by first-generation ELISA was found in 136 (29%). Of sera repeatedly reactive for anti-HCV by first-generation ELISA, two-antigen recombinant immunoblot assay (RIBA) was positive in 41 (32%) and four-antigen RIBA was positive in 55 (41%). Four-antigen RIBA was positive in 33 (30%) of the 109 with an OD on ELISA ofor = 2.0 compared with 22 (81%) of the 27 with an OD2.0 (P.001). Anti-HCV detected by four-antigen RIBA was associated with increasing age, black or Hispanic race, and antibody to hepatitis B core antigen. When patients with hepatitis B surface antigen were excluded, elevated alanine aminotransferase was found in 5 (8%) of 63 with a negative RIBA and 13 (28%) of 47 with a positive RIBA (P = .006). While RIBA was negative in more than half of those with anti-HCV by ELISA, 55 (12%) of these HIV-1 infected personnel had anti-HCV detected by RIBA, which was associated with a strong reaction by ELISA, elevated liver enzymes, coinfection with hepatitis B, minority race, and older age. |
| Databáze: | OpenAIRE |
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