Matrix Metalloproteinases-14, -9 and -2 are Localized to the Podosome and Involved in Podosome Development in the A7r5 Smooth Muscle Cell
| Autor: | Hideyuki Tanaka, Gary L. Wright, Kazuhiro Kohama, Lisa A. Cassis, Mike E. Fultz, Jason Edward Black, Sean E. Thatcher |
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| Rok vydání: | 2017 |
| Předmět: |
0303 health sciences
Podosome Chemistry Cell Extracellular macromolecular substances 030204 cardiovascular system & hematology Matrix metalloproteinase Matrix (biology) Actin cytoskeleton Article Remodeling Cell biology Degradation 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Phorbol cardiovascular system medicine Cytoskeleton Cell adhesion Actin 030304 developmental biology |
| Zdroj: | Journal of cardiobiology |
| ISSN: | 2332-3671 |
| Popis: | Aim The purpose of the study was to localize matrix metalloproteinase (MMP)-14, -9, and -2 in the A7r5 smooth muscle cell and to understand the interaction between these MMPs and the cytoskeleton. This interaction was observed under non-stimulating and phorbol 12, 13-dibutyrate (PDBu)-stimulating conditions. Methods Confocal microscopy was utilized to define the localizations of MMPs and tissue inhibitor of matrix metalloproteinases (TIMPs) in the A7r5 cell and to determine interaction between MMPs and the cytoskeleton. Under PDBu-stimulating conditions, the presence of MMP active forms and activity by gel zymography was evaluated in the A7r5 cell. Actin and microtubule-polymerization inhibitors were used to evaluate MMP interaction with the cytoskeleton and the cytoskeleton was observed on matrix and within a Type I collagen gel. Results MMP-14, -9, and -2 were localized to the podosome in the A7r5 smooth muscle cell and interactions were seen with these MMPs and the actin cytoskeleton. PDBu-stimulation induced increases in the protein abundance of the active forms of the MMPs and MMP-2 activity was increased. MMPs also interact with a-actin and not β-tubulin in the A7r5 cell. Galardin, also known as GM-6001, was shown to inhibit podosome formation and prevented MMP localization to the podosome. This broad spectrum MMP inhibitor also prevented collagen gel contraction and prevented cell adhesion and spreading of A7r5 cells within this collagen matrix. Conclusion MMPs are important in the formation and function of podosomes in the A7r5 smooth muscle cell. MMPs interact with a-actin and not β-tubulin in the A7r5 cell. Podosomes play an important role in cell migration and understanding the function of podosomes can lead to insights into cancer metastasis and cardiovascular disease. |
| Databáze: | OpenAIRE |
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