Testosterone Regulates NUCB2 mRNA Expression in Male Mouse Hypothalamus and Pituitary Gland
| Autor: | Yiwa Chung, Daun Jeon, Heejeong Kim, Hyunwon Yang, Sojeong Seon, Narae Choi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pituitary gland NUCB2 media_common.quotation_subject Hypothalamus 030209 endocrinology & metabolism Hypothalamic–pituitary–gonadal axis Ovary Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Testosterone media_common Appetite Original Research Paper Blot 030104 developmental biology Endocrinology medicine.anatomical_structure Castration Pituitary chemistry Nesfatin-1 |
| Zdroj: | Development & Reproduction |
| ISSN: | 2465-9541 2465-9525 |
| DOI: | 10.12717/dr.2017.21.1.071 |
| Popis: | Nesfatin-1/NUCB2 is known to take part in the control of the appetite and energy metabolism. Recently, many reports have shown nesfatin-1/NUCB2 expression and function in various organs. We previously demonstrated that nesfatin-1/NUCB2 expression level is higher in the pituitary gland compared to other organs and its expression is regulated by 17β-estradiol and progesterone secreted from the ovary. However, currently no data exist on the expression of nesfatin-1/NUCB2 and its regulation mechanism in the pituitary of male mouse. Therefore, we examined whether nesfatin-1/NUCB2 is expressed in the male mouse pituitary and if its expression is regulated by testosterone. As a result of PCR and western blotting, we found that a large amount of nesfatin-1/NUCB2 was expressed in the pituitary and hypothalamus. The NUCB2 mRNA expression level in the pituitary was decreased after castration, but not in the hypothalamus. In addition, its mRNA expression level in the pituitary was increased after testosterone treatment in the castrated mice, whereas, the expression level in the hypothalamus was significantly decreased after the treatment with testosterone. The in vitro experiment to elucidate the direct effect of testosterone on NUCB2 mRNA expression showed that NUCB2 mRNA expression was significantly decreased with testosterone in cultured hypothalamus tissue, but increased with testosterone in cultured pituitary gland. The present study demonstrated that nesfatin-1/NUCB2 was highly expressed in the male mouse pituitary and was regulated by testosterone. This data suggests that reproductive-endocrine regulation through hypothalamus-pituitary-testis axis may contribute to NUCB2 mRNA expression in the mouse hypothalamus and pituitary gland. |
| Databáze: | OpenAIRE |
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