Astrocyte layers in the mammalian cerebral cortex revealed by a single-cell in situ transcriptomic map
| Autor: | Matthew Holt, John H. Stockley, Riki Kawaguchi, Araks Martirosyan, Lucile Ben Haim, Martin Hemberg, Peter J. Hutchinson, Vitalii Kleshchevnikov, Khalida Sabeur, Kirti Prakash, Mykhailo Y. Batiuk, Theresa Bartels, Adam Young, Daniel H. Geschwind, Eric J. Huang, Staffan Holmqvist, Kenny Roberts, Omer Ali Bayraktar, Mercedes F. Paredes, David H. Rowitch, Sandra Chang, Giovanni Coppola, Damon Polioudakis, Erik M. Ullian, Alexander Brown |
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| Přispěvatelé: | Bayraktar, Omer Ali [0000-0001-6055-277X], Holmqvist, Staffan [0000-0001-6709-6666], Kleshchevnikov, Vitalii [0000-0001-9110-7441], Batiuk, Mykhailo Y [0000-0001-6681-4048], Roberts, Kenny [0000-0001-6155-0821], Stockley, John H [0000-0002-7385-8310], Huang, Eric [0000-0002-5381-3801], Hemberg, Martin [0000-0001-8895-5239], Holt, Matthew G [0000-0002-8958-4027], Geschwind, Daniel H [0000-0003-2896-3450], Rowitch, David H [0000-0002-0079-0060], Apollo - University of Cambridge Repository |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Candidate gene 1.1 Normal biological development and functioning Biology Article Transcriptome 03 medical and health sciences Mice 0302 clinical medicine Reeler Underpinning research Cortex (anatomy) Gene expression medicine Genetics Psychology Animals Humans Cerebral Cortex Neurons Brain Mapping Neurology & Neurosurgery General Neuroscience Human Genome Neurosciences 3. Good health Brain Disorders 030104 developmental biology medicine.anatomical_structure Cerebral cortex Astrocytes Neurological Excitatory postsynaptic potential Cognitive Sciences Neuroscience 030217 neurology & neurosurgery Astrocyte Biotechnology |
| Zdroj: | Nat Neurosci Nature neuroscience, vol 23, iss 4 Nature Neuroscience |
| ISSN: | 1546-1726 |
| Popis: | Although the cerebral cortex is organized into six excitatory neuronal layers, it is unclear whether glial cells show distinct layering. In the present study, we developed a high-content pipeline, the large-area spatial transcriptomic (LaST) map, which can quantify single-cell gene expression in situ. Screening 46 candidate genes for astrocyte diversity across the mouse cortex, we identified superficial, mid and deep astrocyte identities in gradient layer patterns that were distinct from those of neurons. Astrocyte layer features, established in the early postnatal cortex, mostly persisted in adult mouse and human cortex. Single-cell RNA sequencing and spatial reconstruction analysis further confirmed the presence of astrocyte layers in the adult cortex. Satb2 and Reeler mutations that shifted neuronal post-mitotic development were sufficient to alter glial layering, indicating an instructive role for neuronal cues. Finally, astrocyte layer patterns diverged between mouse cortical regions. These findings indicate that excitatory neurons and astrocytes are organized into distinct lineage-associated laminae. ispartof: NATURE NEUROSCIENCE vol:23 issue:4 pages:500-+ ispartof: location:United States status: published |
| Databáze: | OpenAIRE |
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