Structural characterization, antioxidant and antibacterial activities of two heteropolysaccharides purified from the bulbs of Lilium davidii var. unicolor Cotton
| Autor: | Xiaoyan Yang, Xiuzhuang Li, Hui Jin, Aiyi Xin, Ruiming Zhao, Heping Hui, Bo Qin |
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| Rok vydání: | 2019 |
| Předmět: |
Arabinose
DPPH Mannose 02 engineering and technology Polysaccharide Biochemistry 03 medical and health sciences chemistry.chemical_compound Structural Biology Polysaccharides Lilium davidii Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Plant Stems Monosaccharides General Medicine Free Radical Scavengers 021001 nanoscience & nanotechnology biology.organism_classification Congo red Anti-Bacterial Agents Molecular Weight chemistry Galactose Lilium 0210 nano-technology Antibacterial activity Nuclear chemistry |
| Zdroj: | International journal of biological macromolecules. 133 |
| ISSN: | 1879-0003 |
| Popis: | Two heteropolysaccharides, BHP-1 and BHP-2, were separated and purified from the edible bulbs of Lilium davidii var. unicolor Cotton by anion-exchange and gel-permeation chromatography. The primary structural characteristics of BHP-1 and BHP-2 were investigated by Congo red test, X-ray diffractometry (XRD) analysis, IR, GC and NMR. The results showed that the weight-average molecular weight (MW) and number-average molecular weight (Mn) of BHP-1 and BHP-2 were nearly 1.93 × 105 g/moL, 8.86 × 104 g/moL and 3.52 × 104 g/moL, 2.95 × 104 g/moL, respectively. Glucose and mannose comprised BHP-1 in a relative molar ratio of 5.9:2.0, and BHP-2 was consisted of glucose, galactose, mannose and arabinose with approximate molar ratios of 8.3:1.5:1.0:1.1. Their backbone mainly contained α-(1 → 4)-linked d -glucopyranosyl. In addition, the C-6 of the glucose and C-2 of mannose residue of BHP-1 and BHP-2 were substituted. The two polysaccharides were semi-crystalline substance with multi-branching structure and triple helix conformation. Bioassays showed that BHP-1 and BHP-2 had effective antioxidant activities evaluated by DPPH, scavenging superoxide radical and OH radical in a dose-dependent manner. The two heteropolysaccharides also displayed distinct and selective antibacterial activity against B. cereus, P. putida, M. luteus and K. pneumonia with inhibition zones of 13 mm, 16 mm, 17 mm, 23 mm and 12 mm, 17 mm, 19 mm, 20 mm, respectively. These findings provided a reference for potential applications of the polysaccharides from L. davidii as natural antioxidants and antibacterial agents in food and pharmaceuticals. |
| Databáze: | OpenAIRE |
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