Coaggregation between probiotic bacteria and caries-associated strains: Anin vitrostudy
| Autor: | Christina Stecksén-Blicks, Lisa Twetman, Nils-Erik Fiehn, Svante Twetman, Ulla Larsen |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Limosilactobacillus reuteri
Time Factors Dental Caries Biology Bacterial Adhesion Microbiology Streptococcus mutans Lactobacillus Escherichia coli Humans In vitro study Probiotic bacteria Anaerobiosis General Dentistry Streptococcus sobrinus Bacteriological Techniques Lacticaseibacillus rhamnosus Probiotics General Medicine Hydrogen-Ion Concentration biology.organism_classification Coculture Techniques In vitro Lactobacillus acidophilus Spectrophotometry Coculture Technique Lactobacillus plantarum |
| Zdroj: | Acta Odontologica Scandinavica. 67:284-288 |
| ISSN: | 1502-3850 0001-6357 |
| DOI: | 10.1080/00016350902984237 |
| Popis: | To evaluate the in vitro abilities of probiotic bacteria derived from consumer products to coaggregate with caries-associated mutans streptococci.Six lactobacillus strains (L. acidophilus (CCUG 5917), L. plantarum 299v, L. rhamnosus GG and LB21, L. paracasei F19, L. reuteri PTA5289) were cultivated under anaerobic conditions at 37°C in Man Rogosa Sharpe (MSB) broth for 24 h. Four strains of human streptococci (S. mutans Ingbritt, S. mutans (ATCC 25175), S. mutans GS-5, S. sobrinus (ATCC 33478) were similarly grown in Brain Heart Infusion (BHI) broth. A gastrointestinal pathogen (Escherichia coli) was aerobically cultivated on BHI broth as a positive control. After incubation, the bacteria were aerobically harvested, washed, and suspended in 10 mmol/l phosphate-buffered saline (pH 7.2). The probiotic strains were characterized with the API 50 CH system to confirm their identity. Coaggregation was determined by spectrophotometry in mixtures and bacterial suspensions alone after 1, 2, 4, and 24 h and expressed as the aggregation ratio (%).All probiotic strains showed coaggregation abilities with the oral pathogens and the results were strain specific and dependent on time. S. mutans GS-5 exhibited a significantly higher ability to coaggregate with all the probiotic strains than the other mutans streptococci and E. coli. The differences among the probiotic strains were modest with L. acidophilus being the most prone and L. rhamnosus LB21 the least prone to coaggregate with the oral streptococci.The results demonstrated different abilities of lactobacilli-derived probiotic bacteria to coaggregate with selected oral streptococci. Aggregation assays may be a useful complement for screening of probiotic candidates with possible anti-caries properties. |
| Databáze: | OpenAIRE |
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