The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue

Autor: Rahel Kastli, Hanns Ulrich Zeilhofer, Evgenia Platonova, Ladan Egolf, Stéphane Pagès, Laura Batti, Karen Haenraets, Paola Perin, Alexander van der Bourg, Thomas Topilko, Christian Lüscher, Theofanis Karayannis, Botond Roska, Daniel Kirschenbaum, Noémie Frezel, Urs Ziegler, Fabian F. Voigt, Anna Schueth, Sven Hildebrand, Philipp Bethge, Alard Roebroeck, Roberto Pizzala, Nicolas Renier, Martina Schaettin, Esther T. Stoeckli, Robert A. A. Campbell, Adriano Aguzzi, Daniel Hillier, Fritjof Helmchen, Anthony Holtmaat
Přispěvatelé: University of Zurich, Voigt, Fabian F, RS: FPN CN 11, Multiscale Imaging of Brain Connectivity
Rok vydání: 2019
Předmět:
Zdroj: Nature Methods
Nature Methods, Vol. 16, No 11 (2019) pp. 1105-1108
Nature Methods, 16(11), 1105-1108. Nature Publishing Group
Nature methods
ISSN: 1548-7105
1548-7091
DOI: 10.1038/s41592-019-0554-0
Popis: Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy (mesoSPIM) initiative, an open-hardware project for building and operating a light sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample (www.mesospim.org).
Databáze: OpenAIRE
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