Intestinal Threonine Utilization for Protein and Mucin Synthesis Is Decreased in Formula-Fed Preterm Pigs
| Autor: | Mette Schmidt, Patrycja J. Puiman, Barbara J. Stoll, Adrianus C. J. M. de Bruijn, Kristien Dorst, Douglas G. Burrin, Per T. Sangild, Henk Schierbeek, Johannes B. van Goudoever, Ingrid B. Renes, Mikkel Jensen, Günther Boehm |
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| Přispěvatelé: | Pediatrics, Pediatric surgery, ICaR - Ischemia and repair, Other departments, General Paediatrics, Neonatology |
| Rok vydání: | 2011 |
| Předmět: |
Threonine
medicine.medical_specialty Sus scrofa Medicine (miscellaneous) Enteral administration Enterocolitis Necrotizing Pregnancy Risk Factors Suidae Internal medicine medicine Animals Humans Intestinal Mucosa Infant Nutritional Physiological Phenomena Essential amino acid chemistry.chemical_classification Mucin-2 Nutrition and Dietetics biology Colostrum Infant Newborn biology.organism_classification medicine.disease Infant Formula digestive system diseases Small intestine Intestines Parenteral nutrition medicine.anatomical_structure Endocrinology Animals Newborn chemistry Protein Biosynthesis Models Animal Necrotizing enterocolitis Animal Nutritional Physiological Phenomena Cattle Female Infant Premature |
| Zdroj: | Journal of Nutrition, 141, 1306-1311. American Society for Nutrition The Journal of nutrition, 141(7), 1306-1311. American Society for Nutrition Journal of nutrition, 141(7), 1306-1311. American Society for Nutrition Puiman, P J, Jensen, M, Stoll, B, Renes, I B, de Bruijn, A C J M, Dorst, K, Schierbeek, H, Schmidt, M, Boehm, G, Burrin, D G, Sangild, P T & van Goudoever, J B 2011, ' Intestinal Threonine Utilization for Protein and Mucin Synthesis Is Decreased in Formula-Fed Preterm Pigs ', The Journal of nutrition, vol. 141, no. 7, pp. 1306-1311 . https://doi.org/10.3945/jn.110.135145 |
| ISSN: | 0022-3166 |
| DOI: | 10.3945/jn.110.135145 |
| Popis: | Threonine is an essential amino acid necessary for synthesis of intestinal (glyco)proteins such as mucin MUC2 to maintain adequate gut barrier function. In premature infants, reduced barrier function may contribute to the development of necrotizing enterocolitis (NEC). Human milk protects against NEC compared with infant formula. Therefore, we hypothesized that formula feeding decreases the MUC2 synthesis rate concomitant with a decrease in intestinal first-pass threonine utilization, predisposing the preterm neonate to NEC. Preterm pigs were delivered by caesarian section and received enteral feeding with formula (FORM; n = 13) or bovine colostrum (COL; n = 6) for 2 d following 48 h of total parenteral nutrition. Pigs received a dual stable isotope tracer infusion of threonine to determine intestinal threonine kinetics. NEC developed in 38% of the FORM pigs, whereas none of the COL pigs were affected (P = 0.13). Intestinal fractional first-pass threonine utilization was lower in FORM pigs (49 6 2%) than in COL pigs (60 6 4%) (P = 0.02). In FORM pigs compared with COL pigs, protein synthesis (369 6 31 mg·kg 21 ·d 21 vs. 615 6 54 mg·kg 21 ·d 21 ; P = 0.003) and MUC2 synthesis (121 6 17%/d vs. 184 6 15%/d; P = 0.02) were lower in the distal small intestine (SI). Our results suggest that formula feeding compared with colostrum feeding in preterm piglets reduces mucosal growth with a concomitant decrease in first-pass splanchnic threonine utilization, protein synthesis, and MUC2 synthesis in the distal SI. Hence, decreased intestinal threonine metabolism and subsequently impaired gut barrier function may predispose the formula-fed infant to developing NEC. J. Nutr. 141: 1306‐1311, 2011. |
| Databáze: | OpenAIRE |
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