Genetic Analysis of Walnut (Juglans regia L.) Pellicle Pigment Variation Through a Novel, High-Throughput Phenotyping Platform
| Autor: | David B. Neale, Gina M. Sideli, Peter A. McAtee, Abhaya M. Dandekar, Patrick J. Brown, Timothy S Butterfield, Annarita Marrano, Charles A. Leslie, Brian J. Allen |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Candidate gene walnut Population Genome-wide association study Juglans computer vision system QH426-470 Quantitative trait locus Biology 01 natural sciences Genetic analysis 03 medical and health sciences Pigment Genetics education Molecular Biology Genetics (clinical) 030304 developmental biology 0303 health sciences education.field_of_study Pigmentation Human Genome Chromosome Mapping bi-parental population biology.organism_classification Phenotype visual_art seed coat Trait visual_art.visual_art_medium genome-wide association 010606 plant biology & botany Genome-Wide Association Study |
| Zdroj: | G3 (Bethesda, Md.), vol 10, iss 12 G3: Genes, Genomes, Genetics, Vol 10, Iss 12, Pp 4411-4424 (2020) |
| Popis: | Walnut pellicle color is a key quality attribute that drives consumer preference and walnut sales. For the first time a high-throughput, computer vision-based phenotyping platform using a custom algorithm to quantitatively score each walnut pellicle in L* a* b* color space was deployed at large-scale. This was compared to traditional qualitative scoring by eye and was used to dissect the genetics of pellicle pigmentation. Progeny from both a bi-parental population of 168 trees (‘Chandler’ × ‘Idaho’) and a genome-wide association (GWAS) with 528 trees of the UC Davis Walnut Improvement Program were analyzed. Color phenotypes were found to have overlapping regions in the ‘Chandler’ genetic map on Chr01 suggesting complex genetic control. In the GWAS population, multiple, small effect QTL across Chr01, Chr07, Chr08, Chr09, Chr10, Chr12 and Chr13 were discovered. Marker trait associations were co-localized with QTL mapping on Chr01, Chr10, Chr14, and Chr16. Putative candidate genes controlling walnut pellicle pigmentation were postulated. |
| Databáze: | OpenAIRE |
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