New tools to screen wild peanut species for aflatoxin accumulation and genetic fingerprinting

Autor: Naveen Puppala, Francisco Javier De Blas, Victor S. Sobolev, Brian E. Scheffler, Valerie A. Orner, Guillermo J. Seijo, Sheron A. Simpson, Renee S. Arias, Travis Walk, Alicia N. Massa, Linda L. Ballard
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: BMC Plant Biology
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
BMC Plant Biology, Vol 18, Iss 1, Pp 1-13 (2018)
ISSN: 1471-2229
Popis: Aflatoxin contamination in peanut seeds is still a serious problem for the industry and human health. No stable aflatoxin resistant cultivars have yet been produced, and given the narrow genetic background of cultivated peanuts, wild species became an important source of genetic diversity. Wild peanut seeds, however, are not abundant, thus, an effective method of screening for aflatoxin accumulation using minimal seeds is highly desirable. In addition, keeping record of genetic fingerprinting of each accession would be very useful for breeding programs and for the identification of accessions within germplasm collections. Results: In this study, we report a method of screening for aflatoxin accumulation that is applicable to the small-size seeds of wild peanuts, increases the reliability by testing seed viability, and records the genetic fingerprinting of the samples. Aflatoxin levels observed among 20 wild peanut species varied from zero to 19000 ng.g-1 and 155 ng.g-1 of aflatoxin B1 and B2, respectively. We report the screening of 373 molecular markers, including 288 novel SSRs, tested on 20 wild peanut species. Multivariate analysis by Neighbor-Joining, Principal Component Analysis and 3D-Principal Coordinate Analysis using 134 (36 %) transferable markers, in general grouped the samples according to their reported genomes. The best 88 markers, those with high fluorescence, good scorability and transferability, are reported with BLAST results. High quality markers (total 98) that discriminated genomes are reported. A high quality marker with UPIC score 16 (16 out of 20 species discriminated) had significant hits on BLAST2GO to a pentatricopeptide-repeat protein, another marker with score 5 had hits on UDP-D-apiose synthase, and a third one with score 12 had BLASTn hits on La-RP 1B protein. Together, these three markers discriminated all 20 species tested. Conclusions: This study provides a reliable method to screen wild species of peanut for aflatoxin resistance using minimal seeds. In addition we report 288 new SSRs for peanut, and a cost-effective combination of markers sufficient to discriminate all 20 species tested. These tools can be used for the systematic search of aflatoxin resistant germplasm keeping record of the genetic fingerprinting of the accessions tested for breeding purpose. Fil: Arias, Renee S.. National Peanut Research Laboratory; Estados Unidos Fil: Sobolev, Victor S.. National Peanut Research Laboratory; Estados Unidos Fil: Massa, Alicia N.. National Peanut Research Laboratory; Estados Unidos Fil: Orner, Valerie A.. National Peanut Research Laboratory; Estados Unidos Fil: Walk, Travis E.. National Peanut Research Laboratory; Estados Unidos Fil: Ballard, Linda L.. Usda Agricultural Research Service; Estados Unidos Fil: Simpson, Sheron A.. Usda Agricultural Research Service; Estados Unidos Fil: Puppala, Naveen. New Mexico State University Las Cruces; México Fil: Scheffler, Brian E.. Usda Agricultural Research Service; Estados Unidos Fil: de Blas, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Databáze: OpenAIRE
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