Root hairs play a key role in the endophytic colonization of olive roots by Pseudomonas spp. with biocontrol activity
| Autor: | María Mercedes Maldonado-González, Juan Bautista Barroso-Albarracín, Pilar Prieto, Elisabetta Schilirò, Raquel Valderrama, Jesús Mercado-Blanco |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Microscopy
Confocal Ecology biology Pseudomonas Soil Science food and beverages Pseudomonas fluorescens Fungus Root hair Agricultural Inoculants biology.organism_classification Plant Roots Pseudomonas putida Luminescent Proteins Biological Control Agents Olea Botany Endophytes Colonization Verticillium dahliae Verticillium wilt Plant Microbe Interactions Ecology Evolution Behavior and Systematics |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Microbial Ecology |
| Popis: | 11 pages; 3 figures The use of indigenous bacterial root endophytes with biocontrol activity against soil-borne phytopathogens is an environmentally-friendly and ecologically-efficient action within an integrated disease management framework. The earliest steps of olive root colonization by Pseudomonas fluorescens PICF7 and Pseudomonas putida PICP2, effective biocontrol agents (BCAs) against Verticillium wilt of olive (Olea europaea L.) caused by the fungus Verticillium dahliae Kleb., are here described. A gnotobiotic study system using in vitro propagated olive plants, differential fluorescent-protein tagging of bacteria, and confocal laser scanning microscopy analysis have been successfully used to examine olive roots–Pseudomonas spp. interactions at the single-cell level. In vivo simultaneous visualization of PICF7 and PICP2 cells on/in root tissues enabled to discard competition between the two bacterial strains during root colonization. Results demonstrated that both BCAs are able to endophytically colonized olive root tissues. Moreover, results suggest a pivotal role of root hairs in root colonization by both biocontrol Pseudomonas spp. However, colonization of root hairs appeared to be a highly specific event, and only a very low number of root hairs were effectively colonized by introduced bacteria. Strains PICF7 and PICP2 can simultaneously colonize the same root hair, demonstrating that early colonization of a given root hair by one strain did not hinder subsequent attachment and penetration by the other. Since many environmental factors can affect the number, anatomy, development, and physiology of root hairs, colonization competence and biocontrol effectiveness of BCAs may be greatly influenced by root hair’s fitness. Finally, the in vitro study system here reported has shown to be a suitable tool to investigate colonization processes of woody plant roots by microorganisms with biocontrol potential. Grants P07-CVI- 02624 (Proyecto de Excelencia, Junta de Andalucía, Spain) and AGL2009-07275 (Spanish Ministerio de Ciencia e Innovación, cofinanced by FEDER of the UE). |
| Databáze: | OpenAIRE |
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