Transcription of meiotic-like-pathway genes in Giardia intestinalis
| Autor: | Amanda Gallego, Vanessa Gómez, Moisés Wasserman, Sandra Melo, Paula C. Hernández, Isabel C Castellanos, Magda E. Alvarado |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
dmc1 lcsh:Arctic medicine. Tropical medicine Spo11 Transcription Genetic lcsh:RC955-962 Genes Protozoan lcsh:QR1-502 medicine.disease_cause Genome lcsh:Microbiology Loss of heterozygosity hop1 Meiosis Reproduction Asexual medicine Animals meiosis Giardia lamblia Crossing Over Genetic RNA Messenger Gene spo11 Genetics biology Reverse Transcriptase Polymerase Chain Reaction Giardia Giardia intestinalis biology.organism_classification biology.protein DMC1 |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 103, Iss 4, Pp 347-350 (2008) Memórias do Instituto Oswaldo Cruz, Volume: 103, Issue: 4, Pages: 347-350, Published: JUN 2008 |
| ISSN: | 0074-0276 |
| DOI: | 10.1590/s0074-02762008000400006 |
| Popis: | The reproductive mechanism of Giardia intestinalis, considered one of the earliest divergent eukaryotes, has not been fully defined yet. Some evidence supports the hypothesis that Giardia is an exclusively asexual organism with a clonal population structure. However, the high genetic variability, the variation in ploidy during its life cycle, the low heterozygosity and the existence of genes involved in the meiotic-like recombination pathway in the parasite's genome cast doubt on exclusively asexual nature of Giardia. In this work, semiquantitative RT-PCR analysis was used to assess the transcription pattern of three meiosis-like-specific genes involved in homologues recombination: dmc1, hop1 and spo11. The mRNAs were amplified during the parasite's differentiation processes, encystation and excystation, and expression was found at each stage of its life cycle. A semiquantitative assessment also suggests that expression of some of the genes is regulated during encystation process. |
| Databáze: | OpenAIRE |
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