HIV Type 1 Subtype Distribution, Multiple Infections, Sexual Networks, and Partnership Histories in Female Sex Workers in Kampala, Uganda
| Autor: | Fred Lyagoba, Janet Seeley, Heiner Grosskurth, Pontiano Kaleebu, Judith Vandepitte, Deogratius Ssemwanga, Nicaise Ndembi, Justine Bukenya |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
medicine.medical_specialty Genotype Molecular Sequence Data Immunology Population Prevalence Polymerase Chain Reaction Cohort Studies Risk-Taking Surveys and Questionnaires Virology HIV Seropositivity Epidemiology medicine Humans Uganda education Sex work Molecular Epidemiology education.field_of_study Sex Workers AIDS-Related Opportunistic Infections Molecular epidemiology business.industry Subtyping Sexual Partners Infectious Diseases Cohort HIV-1 RNA Viral Female business Demography Cohort study |
| Zdroj: | AIDS Research and Human Retroviruses. 28:357-365 |
| ISSN: | 1931-8405 0889-2229 |
| DOI: | 10.1089/aid.2011.0024 |
| Popis: | We investigated for the first time the subtype distribution, prevalence of multiple HIV-1 infections, sexual networks, and partnership histories in a cohort of women engaged in high-risk sexual behavior such as female sex workers (FSWs) and women employed in entertainment facilities. Viral RNA was extracted from blood samples collected from 324 HIV-1-positive women; the gp-41 and pol-IN genes were directly sequenced. Women found to have closely related viruses and those with recombinant viruses were further analyzed in the pol-IN gene by clonal sequencing to determine HIV-1 multiple infections. Individual partnership histories were used to provide information on when sex work was undertaken and where. Subtyping in both gp-41 and pol-IN was successfully done in 210/324 (64.8%) women. Subtype distribution in these two genes was 54.3% (n=114) A/A, 2.9% (n=6) C/C, 24.3% (n=51) D/D, 11.9% (n=25) A/D, 4.8% (n=10) D/A, 0.5% (n=1) C/A, 1.0% (n=2) B/A, and 0.5% (n=1) B/D. Sexual networks were identified in six pairs and one triplet of women with closely related subtype A viruses. Partnership histories showed that women having phylogenetically similar viruses had worked in the same localities. Five cases of multiple infections were confirmed: four dual infections and one triple infection. In this first molecular epidemiology study among FSWs in Kampala, subtype A was the predominant subtype. About 9% of a subgroup had multiple infections. Partnership histories and multiple infections observed in this population suggest sexual mixing of the FSWs and their clients confirming their high-risk characteristics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|