The Importance of Evaluating the Lot-to-Lot Batch Consistency of Commercial Multi-Walled Carbon Nanotube Products
| Autor: | Alexander T. Brown, Kenneth J. Balkus, Shashini D. Diwakara, Mai T. Huynh, Paul Pantano, Samad Yousuf, Ronald A. Smaldone, Rockford K. Draper, Jean François Veyan, Hong Pham, Jason Lin, Raina Rahman, Carole Mikoryak, Bryanna LeGrand |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
General Chemical Engineering
02 engineering and technology Carbon nanotube 010402 general chemistry 01 natural sciences Article law.invention lcsh:Chemistry law General Materials Science Carbon nanomaterials carbon nanomaterials Surface oxygen Chemistry engineered nanoparticles 021001 nanoscience & nanotechnology Engineered nanoparticles nanomedicine 0104 chemical sciences macrophages lcsh:QD1-999 Chemical engineering Nanotoxicology nanotoxicity 0210 nano-technology Potential toxicity |
| Zdroj: | Nanomaterials Volume 10 Issue 10 Nanomaterials, Vol 10, Iss 1930, p 1930 (2020) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano10101930 |
| Popis: | The biological response of multi-walled carbon nanotubes (MWNTs) is related to their physicochemical properties and a thorough MWNT characterization should accompany an assessment of their biological activity, including their potential toxicity. Beyond characterizing the physicochemical properties of MWNTs from different sources or manufacturers, it is also important to characterize different production lots of the same MWNT product from the same vendor (i.e., lot-to-lot batch consistency). Herein, we present a comprehensive physicochemical characterization of two lots of commercial pristine MWNTs (pMWNTs) and carboxylated MWNTs (cMWNTs) used to study the response of mammalian macrophages to MWNTs. There were many similarities between the physicochemical properties of the two lots of cMWNTs and neither significantly diminished the 24-h proliferation of RAW 264.7 macrophages up to the highest concentration tested (200 &mu g cMWNTs/mL). Conversely, several physicochemical properties of the two lots of pMWNTs were different notably, the newer lot of pMWNTs displayed less oxidative stability, a higher defect density, and a smaller amount of surface oxygen species relative to the original lot. Furthermore, a 72-h half maximal inhibitory concentration (IC-50) of ~90 µ g pMWNTs/mL was determined for RAW 264.7 cells with the new lot of pMWNTs. These results demonstrate that subtle physicochemical differences can lead to significantly dissimilar cellular responses, and that production-lot consistency must be considered when assessing the toxicity of MWNTs. |
| Databáze: | OpenAIRE |
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