A live-imaging protocol to track cell movement in the Xenopus embryo
| Autor: | Fabrice Daian, Andrea Pasini, Laurent Kodjabachian, Alexandre Chuyen |
|---|---|
| Přispěvatelé: | Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Embryo
Nonmammalian Science (General) [SDV]Life Sciences [q-bio] Xenopus Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Xenopus laevis Q1-390 0302 clinical medicine Model Organisms Live cell imaging Cell Movement Developmental biology Protocol Image Processing Computer-Assisted Animals Protocol (object-oriented programming) 030304 developmental biology 0303 health sciences Microscopy Microscopy Video General Immunology and Microbiology General Neuroscience Track (disk drive) Tissue explants Organisms Embryo Cell movement Cell Biology biology.organism_classification Cell biology Luminescent Proteins Cell Tracking Cell 030217 neurology & neurosurgery Model |
| Zdroj: | STAR Protocols, Vol 2, Iss 4, Pp 100928-(2021) STAR Protocols STAR Protocols, Elsevier, 2021, 2 (4), pp.100928. ⟨10.1016/j.xpro.2021.100928⟩ STAR Protocols, 2021, 2 (4), pp.100928. ⟨10.1016/j.xpro.2021.100928⟩ |
| ISSN: | 2666-1667 |
| DOI: | 10.1016/j.xpro.2021.100928⟩ |
| Popis: | Summary Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells (MCCs) moving within the inner epidermal layer of a whole embryo. In addition, we present a data processing protocol to uncouple the movements of individual cells from the coplanar drifts of the tissue in which they are embedded. For complete details on the use and execution of this protocol, please refer to Chuyen et al. (2021). Graphical abstract Highlights • Tracking of individual cell movement in vivo during Xenopus embryonic development • Labeling and tracking of multiciliated cells (MSSs) in bilayered Xenopus epidermis • In silico stabilization corrects drifting caused by embryo morphogenesis Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells (MCCs) moving within the inner epidermal layer of a whole embryo. In addition, we present a data processing protocol to uncouple the movements of individual cells from the coplanar drifts of the tissue in which they are embedded. |
| Databáze: | OpenAIRE |
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