C-deletion in exon 4 codon 63 of p53 gene as a molecular marker for oral squamous cell carcinoma: A preliminary study
| Autor: | Rajkumar Krishnan, Hemani Sukhija, Karthik Raghavendhar, Ramya Ramadoss, N Balachander, Sukanta Sen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Saliva
polymerase chain reaction Orthodontics Biology medicine.disease_cause law.invention Exon law medicine Gene Polymerase chain reaction Mutation p53 C-deletion oral cancer Molecular biology oral squamous cell carcinoma lcsh:RK1-715 genomic DNA stomatognathic diseases lcsh:Dentistry Agarose gel electrophoresis Cancer cell Periodontics Original Article Oral Surgery |
| Zdroj: | Contemporary Clinical Dentistry, Vol 6, Iss 6, Pp 227-234 (2015) Contemporary Clinical Dentistry |
| ISSN: | 0976-2361 |
| Popis: | Background: Exfoliated oral cancer cells in saliva samples from patients with oral squamous cell carcinoma (OSCC) can be used to determine the incidence and type of mutations of the p53 tumor suppressor gene. The purpose of this study was to identify C-deletion mutation in exon 4 codon 63 of p53 gene in the saliva of OSCC patients by polymerase chain reaction (PCR). Materials and Methods: Saliva samples of 20 newly histopathologically diagnosed OSCC patients and 5 healthy volunteers were subjected to isolation of the total genomic DNA and PCR amplification for C-deletion on exon 4 of p53 gene. The resulting products were resolved by agarose gel electrophoresis, viewed and photographed on ultraviolet-transilluminator. Results: The relationship between the frequencies of genetic alterations was assessed by Chi-square test. Differences with values of P < 0.05 were statistically significant. Conclusion: The study concluded a 100% presence of C-deletion mutation in exon 4 codon 63 of p53 in the saliva of OSCC patients. This study suggests that detection of mutation in exon 4 codon 63 of p53 by PCR is a fast, reliable, accurate, and sensitive molecular method for OSCC diagnosis. |
| Databáze: | OpenAIRE |
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