| Autor: |
Zaccaria, Edoardo, Klaassen, Tim, Alleleyn, Annick M. E., Boekhorst, Jos, Smokvina, Tamara, Kleerebezem, Michiel, Troost, Freddy J. |
| Rok vydání: |
2023 |
| DOI: |
10.6084/m9.figshare.22617536 |
| Popis: |
Additional file 1: Supplementary Methods. Recruitment criteria, Gastrointestinal permeability, Short Chain Fatty Acids, Intestinal Microbiota composition, Metatranscriptomics, Urine metabolites profiling, Ileostomy effluent bacterial density calculation, Data mining and statistics. Supplementary Results. Gastrointestinal permeability and SCFA. Supplementary Figure 1. Microbial composition profiles comparison. Supplementary Figure S2. Type and severity of gastrointestinal symptoms. Supplementary Figure S3. Short chain fatty acid concentration in ileostomy effluents. Supplementary Figure S4. Gastro-intestinal permeability. Supplementary Figure S5. Short-term gastro-intestinal permeability. Supplementary Figure S6. Overall and longitudinal microbiota composition analysis. Supplementary Figure S7. Beta diversity of ileostomy effluent microbiota, intra and inter subject; Supplementary Figure S8. Carry-over effect analysis. Supplementary Figure S9. Alpha diversity of ileostomy effluent microbiota. Supplementary Figure S10. Beta diversity of the ileostomy effluent microbiota. Supplementary Figure S11. Core-microbiome. Supplementary Figure S12. L. rhamnosus relative abundance and microbial density in ileostomy samples. Figure S13. Inter subject differences functional metatranscriptome mapping analysis. Supplementary Figure S14. Intervention products effects on the functional metatranscriptome mapping of the ileostomy effluent. Supplementary Figure S15. Analysis of the relashionship between Peptostreptococcaceae and urine microbial metabolites generated through bacterial proteolytic fermentation. Supplementary Figure S18. Taxonomic composition at genera level of the Peptostreptococcaceae family per volunteer. Supplementary Figure 20. Strain specific qPCR. Supplementary Figure 21. Strain specific PCR. Supplementary Figure 22. Correlation analysis between the qPCR estimated copies of L. rhamnosus genome and L. rhamnosus relative abundance obtained via 16S compositional analysis. Supplementary Figure 23. L. rhamnosus and S. thermophilus show a similar. Supplementary Figure 24. Correlation between of L. rhamnosus and S. thermophilus. Supplementary Table S1. Protocol deviations. Supplementary Table S2. Demographic characteristics. Supplementary Table S3. Overview of the successful microbiota composition determination. Supplementary Table S4. Successful samples and success rate per trial phase. Supplementary Table S5. Differential abundance analysis, species. Supplementary Table S6. Differential abundance analysis, pathways. Supplementary References. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
