Biochemical characterization of blood group-active glycosphingolipids
| Autor: | J.-F. Bouhours, D. Bouhours |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Magnetic Resonance Spectroscopy
medicine.drug_class Clinical Biochemistry Cell Molecular Sequence Data Carbohydrates Monoclonal antibody Mass spectrometry Glycosphingolipids Mass Spectrometry Blood cell Glycolipid Sphingosine medicine Humans Chromatography Chemistry Biochemistry (medical) Antibodies Monoclonal Hematology Sphingolipid Red blood cell Membrane medicine.anatomical_structure Biochemistry Carbohydrate Sequence Blood Group Antigens Immunologic Techniques lipids (amino acids peptides and proteins) |
| Zdroj: | Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine. 2(4) |
| ISSN: | 1246-7820 |
| Popis: | Summary Glycosphingolipids are quantitatively minor components of cell lipids. However, their segregation in the outer leaflet of the plasma membrane confers to these membranes specific structural and immunological properties. Current methods of extraction, purification and analysis of blood cell glycolipids are presented. Valuable structural data may be obtained by a combination of chemical and enzymatic degradations with thin-layer chromatography and immunological detection by monoclonal antibodies of known specificity. Examples of physical characterization by Mass Spectrometry and Proton Magnetic Resonance Spectroscopy are also presented. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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