Apolipoprotein E2/E3/E4 genotyping with agarose gels
| Autor: | Geesje M. Dallinga-Thie, M. Van Linde-Sibenius Trip, T.W.A. de Bruin, L. A. W. Kock |
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| Rok vydání: | 1995 |
| Předmět: |
Isoelectric focusing
Biochemistry (medical) Clinical Biochemistry Biology Molecular biology law.invention Apolipoproteins E chemistry.chemical_compound genomic DNA Biochemistry chemistry law Agarose lipids (amino acids peptides and proteins) Apolipoprotein E2 Genotyping Polymerase chain reaction DNA |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/41.1.73 |
| Popis: | Here we report an improved, simple method to assign the human apolipoprotein (apo) E genotype and its isoforms, apo E2, apo E3, and apo E4. Genomic DNA was amplified with specific primers that included the polymorphic region of amino acids 112 and 158. Digestion of the product with Hhal resulted in unique fragments that were separated on Meta-Phor agarose instead of polyacrylamide. The pattern of unique DNA fragments obtained unequivocally characterizes the different apo E alleles. |
| Databáze: | OpenAIRE |
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