HN Protein of Newcastle Disease Virus Induces Apoptosis Through SAPK/JNK Pathway
| Autor: | Shishir Kumar Gupta, Ashok K. Tiwari, Uttara Chaturvedi, Ravi Kumar Gandham, R. S. Rajmani, Aditya Prasad Sahoo, Rajiv Kumar, Shikha Saxena, Prafull Kumar Singh |
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| Rok vydání: | 2015 |
| Předmět: |
MAP Kinase Signaling System
Newcastle disease virus Apoptosis Bioengineering Biology Applied Microbiology and Biotechnology Biochemistry HeLa chemistry.chemical_compound Transactivation Humans HN Protein Molecular Biology c-jun JNK Mitogen-Activated Protein Kinases Interferon-alpha General Medicine Phosphatidylserine biology.organism_classification Up-Regulation Cell biology chemistry DNA fragmentation Hemagglutinin-neuraminidase HeLa Cells Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 177:940-956 |
| ISSN: | 1559-0291 0273-2289 |
| Popis: | Many viral proteins are responsible for causing induction of apoptosis in the target cells. Hemagglutinin neuraminidase (HN), a multifunctional protein of Newcastle disease virus (NDV), is one of such proteins. The present study was undertaken to determine the apoptotic potential of the HN gene in cultured human cervical cancer cell line (HeLa cell) and to elucidate the molecular mechanisms involved. The results of the study indicate that HN protein causes apoptosis in HeLa cells, as observed by the translocation of Phosphatidylserine, activation of caspases, cleavage of poly (ADP-ribose) polymerase (PARP), and DNA fragmentation. Further, we report that expression of HN protein upregulates the SAPK/JNK pathway leading to transactivation of c-Jun which in turn activates apoptosis signaling. The results of our study provide an insight into the mechanism through which HN induces apoptosis. |
| Databáze: | OpenAIRE |
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