In vitro efficacy of ganciclovir against feline herpesvirus type 1 and assessment of ocular tolerability in healthy cats
| Autor: | Renee T. Carter, Chin-Chi Liu, Bruna Miessler, Christopher Alling, Andrew C. Lewin, Pilar Camacho-Luna |
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| Rok vydání: | 2020 |
| Předmět: |
Ganciclovir
040301 veterinary sciences Cat Diseases Antiviral Agents 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Animals Medicine Varicellovirus Small Animals Ocular disease Herpesviridae CATS Feline herpesvirus type 1 business.industry Herpesviridae Infections 04 agricultural and veterinary sciences Virology In vitro Tolerability Cats 030221 ophthalmology & optometry Feline herpesvirus business medicine.drug |
| Zdroj: | Journal of Feline Medicine and Surgery. 23:400-404 |
| ISSN: | 1532-2750 1098-612X |
| Popis: | Objectives Feline herpesvirus-1 (FHV-1) is a prevalent cause of ocular disease in cats and limited topical options for treatment currently exist. The first objective of this study was to confirm the efficacy of ganciclovir against FHV-1 in vitro. The second objective was to assess the safety and ocular tolerability of topically applied ganciclovir eye gel (GEG) in healthy cats. Methods FHV-1 was used to infect tissue culture wells covered in maximally confluent Crandall–Rees feline kidney cells prior to the addition of three molarities of ganciclovir (8.9 µM, 17.8 µM and 89 µM) before being incubated for 48 h. Ganciclovir efficacy in vitro was then assessed using standard plaque reduction assay. Commercially available GEG (0.15%) was applied q8h to one randomly chosen eye of four healthy cats for 7 days. Commercially available lubricating eye gel (LEG) was applied to the opposite eye q8h. Complete blood counts (CBCs), blood chemistry panels (CHEM) and urinalysis (UA) were performed on all cats before and after the study period. Ocular lesions were assessed daily using a standardized scheme. Results Ganciclovir led to a significant reduction in FHV-1 plaque number, area and diameter at all tested molarities in vitro. The highest molarity assessed (89 µM) caused a 100% reduction in viral plaque number. There was no significant difference in ocular lesion scores between eyes receiving GEG and LEG. Animals remained healthy throughout the study period with CBC, CHEM and UA showing no clinically significant alterations. Conclusions and relevance Based on the in vitro results, ganciclovir appears to be effective against FHV-1 in vitro. When applied q8h as a commercial 0.15% gel to a small group of cats with normal eyes, this medication was well tolerated. Taken together, these data suggest this medication warrants further investigation in cats with ocular disease caused by FHV-1. |
| Databáze: | OpenAIRE |
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