Colocalization of Natriuretic Peptide and Estrogen Receptor Immunoreactivities in Preoptic Nuclei in the Female Rat
Autor: | Watson Re, John W. Landis, Lanny C. Keil, Robert K. Hutchinson, David M. Rainey, M. Chris Langub, Shikha Seksaria |
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Rok vydání: | 1994 |
Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Endocrinology Diabetes and Metabolism Hypothalamus Estrogen receptor Biology Rats Sprague-Dawley Cellular and Molecular Neuroscience Endocrinology Atrial natriuretic peptide Internal medicine medicine Animals Endocrine and Autonomic Systems Colocalization Immunohistochemistry Preoptic Area Recombinant Proteins Rats Gonadotropin secretion Preoptic area Receptors Estrogen nervous system Estrogen Female Anteroventral periventricular nucleus Receptors Atrial Natriuretic Factor Atrial Natriuretic Factor hormones hormone substitutes and hormone antagonists |
Zdroj: | Journal of Neuroendocrinology. 6:79-87 |
ISSN: | 1365-2826 0953-8194 |
Popis: | Estrogen is known to play an important role in regulating reproductive function in female rats through actions exerted at the preoptic area, a part of the brain that is markedly sexually dimorphic and which contains abundant estrogen receptors. A critical question to our understanding of estrogen's action on the brain is to identify the types of neurons that contain estrogen receptors (ER). Previous studies have shown that atrial natriuretic peptide (ANP) is in abundance in the preoptic area, and that ANP and other natriuretic peptides are capable of regulating gonadotropin secretion. In an effort to determine whether ERs are present in natriuretic peptide-immunoreactive (NP-ir) neurons in the preoptic area of the rat, double label immunocytochemistry was performed. Since ER-ir, as demonstrated with antibody H222 is known to be localized predominantly in cell nuclei, while NP-ir is present in the cytoplasm, single cells can be double labeled. Diaminobenzidine tetrahydrochloride was used for localization of NP-ir neurons, while nickel-enhanced diaminobenzidine tetrahydrochloride was used for localization of ER-ir. The results revealed that many nuclei throughout the preoptic area contained neurons that were ER-ir or NP-ir and that a substantial number were double labeled. Cell counts in selected preoptic nuclei and components, including the anteroventral periventricular nucleus, periventricular preoptic nucleus, medial part of the medial preoptic nucleus, and central part of the medial preoptic nucleus revealed that 13.6%, 11.1%, 13.5%, and 24.4%, respectively, of the NP-ir neurons in these nuclei also contained ER-ir. Collectively, a total of 14.9% of the NP-ir neurons in these nuclei also contained ER-ir. These results support the hypothesis that estrogen may regulate the activity of NP-ir cells in the preoptic area and thereby may be capable of altering functions associated with NPs in this region of the brain, including not only regulation of gonadotropin secretion, but also control of blood pressure and fluid balance. |
Databáze: | OpenAIRE |
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