LHCSR Expression under HSP70/RBCS2 Promoter as a Strategy to Increase Productivity in Microalgae

Autor: Matteo Ballottari, Federico Perozeni, Giulio Rocco Stella
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
light harvesting
microalgae
non-photochemical quenching
photoprotection
photosynthesis
plant biotechnology
Pigment binding
Light-Harvesting Protein Complexes
Chlamydomonas reinhardtii
Biomass
Photosynthetic efficiency
Photosynthesis
01 natural sciences
Catalysis
Article
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
HSP70 Heat-Shock Proteins
Physical and Theoretical Chemistry
Promoter Regions
Genetic
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
biology
Chemistry
Non-photochemical quenching
Organic Chemistry
RuBisCO
General Medicine
biology.organism_classification
Computer Science Applications
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Photoprotection
biology.protein
Biophysics
010606 plant biology & botany
Biotechnology
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 19, Iss 1, p 155 (2018)
International Journal of Molecular Sciences; Volume 19; Issue 1; Pages: 155
ISSN: 1422-0067
DOI: 10.3390/ijms19010155
Popis: Microalgae are unicellular photosynthetic organisms considered as potential alternative sources for biomass, biofuels or high value products. However, limited biomass productivity is commonly experienced in their cultivating system despite their high potential. One of the reasons for this limitation is the high thermal dissipation of the light absorbed by the outer layers of the cultures exposed to high light caused by the activation of a photoprotective mechanism called non-photochemical quenching (NPQ). In the model organism for green algae Chlamydomonas reinhardtii, NPQ is triggered by pigment binding proteins called light-harvesting-complexes-stress-related (LHCSRs), which are over-accumulated in high light. It was recently reported that biomass productivity can be increased both in microalgae and higher plants by properly tuning NPQ induction. In this work increased light use efficiency is reported by introducing in C. reinhardtii a LHCSR3 gene under the control of Heat Shock Protein 70/RUBISCO small chain 2 promoter in a npq4 lhcsr1 background, a mutant strain knockout for all LHCSR genes. This complementation strategy leads to a low expression of LHCSR3, causing a strong reduction of NPQ induction but is still capable of protecting from photodamage at high irradiance, resulting in an improved photosynthetic efficiency and higher biomass accumulation.
Databáze: OpenAIRE
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