Pectin-encrusted gold nanocomposites containing phytic acid and jacalin: 1,2-dimethylhydrazine-induced colon carcinogenesis in Wistar rats, PI3K/Akt, COX-2, and serum metabolomics as potential targets
| Autor: | Shubhini A. Saraf, Poonam Parashar, Mahendra Singh, Chandra Bhushan Tripathi, Malti Arya, Gaurav Kaithwas, Jovita Kanoujia, Pooja Singh, Krishna P. Gupta, Anupam Guleria |
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| Rok vydání: | 2018 |
| Předmět: |
food.ingredient
Phytic Acid Pectin Drug Compounding Metal Nanoparticles Pharmaceutical Science 02 engineering and technology Oxidative phosphorylation Pharmacology 030226 pharmacology & pharmacy Phosphatidylinositol 3-Kinases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food Chlorides Animals Metabolomics Chelation Rats Wistar Protein kinase B PI3K/AKT/mTOR pathway Phytic acid 021001 nanoscience & nanotechnology Xenograft Model Antitumor Assays Gold Compounds 1 2-Dimethylhydrazine Rats Gene Expression Regulation Neoplastic chemistry Cyclooxygenase 2 Colonic Neoplasms Jacalin Plant Lectins 0210 nano-technology Proto-Oncogene Proteins c-akt |
| Zdroj: | Drug Delivery and Translational Research. 9:53-65 |
| ISSN: | 2190-3948 2190-393X |
| Popis: | Phytic acid (PA) has momentous chemotherapeutic potential. Due to the chelate formation and rapid elimination, it is not popular in cancer treatment. The present work was inquested to develop a surface-modified nanoformulation of PA which prevents its speedy elimination and maximizes chemotherapeutic action. Chloroauric acid was reduced with pectin to produce pectin-gold nanoparticles (PGNPs). PGNPs were incubated with PA and jacalin for drug loading and surface modifications, respectively, to form PA-loaded jacalin-pectin-gold nanoparticles (PA-J-PGNPs). Formulation(s) were assessed for various pharmaceutical/pharmacological parameters. To validate the efficacy against colon carcinogenesis, formulation(s) were assessed in 1,2-dimethylhydrazine (DMH)-treated Wistar rats. DMH treatment distorted colonic architecture, oxidative, and hemodynamic parameters, which were favorably restored by PA-J-PGNP administration. To further confirm our deliberations, formulation(s) were also examined against DMH-altered metabolic changes and expression of markers pertaining to cellular proliferation, which was reinstated by PA-J-PGNPs. Our findings establish PA formulation(s) as a promising approach for suppression of colon carcinogenesis. |
| Databáze: | OpenAIRE |
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