The influence of constitutive cox-2 in smooth muscle tissue on the contractile effect of phenylephrine in the rat abdominal aorta
| Autor: | Carlos Castillo-Henkel, Enrique F. Castillo, Gustavo Guevara-Balcazar, Ruth M. López, Maria del Carmen Castillo-Hernandez, Juan Asbun-Bojalil, Pedro López-Sánchez |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Diacylglycerol lipase Vascular smooth muscle Phospholipase A2 Inhibitors Immunoblotting Indomethacin General Biochemistry Genetics and Molecular Biology Phenylephrine chemistry.chemical_compound Internal medicine medicine.artery medicine Animals Thoracic aorta Cyclooxygenase Inhibitors Aorta Abdominal Cycloheximide Nitrobenzenes Arachidonyl trifluoromethyl ketone Sulfonamides Aorta General Immunology and Microbiology biology Muscle Smooth Rats Rhc80267 Endocrinology Gene Expression Regulation chemistry Cyclooxygenase 2 Type C Phospholipases Cyclooxygenase 1 cardiovascular system biology.protein Pyrazoles Arachidonic acid Muscle Contraction medicine.drug |
| Zdroj: | Frontiers in Bioscience. :441-448 |
| ISSN: | 1945-0508 1945-0494 |
| DOI: | 10.2741/e103 |
| Popis: | Prostanoids are involved in the phenylephrine-induced contraction of the aorta. Here, we examined whether or not constitutive cyclooxygenase-2 (phosholipases C and A2) is the source of prostanoids in the smooth muscle of the arterial wall of the thoracic and abdominal aorta. Both cyclooxygenase isoforms (COX-1 and COX-2) were expressed in the two aortic segments, but their expression was not altered by phenylephrine, the protein synthesis inhibitor cycloheximide, or the phospholipase A2 inhibitors arachidonyl trifluoromethyl ketone and methyl arachidonyl fluorophosponate. Indomethacin and NS398, which are a non-selective and selective COX-2 inhibitor, respectively, but not SC-560, which is a COX-1-selective inhibitor, inhibited the effect of phenylephrine on the abdominal, but not the thoracic, aorta. Similarly, U73122, which is a phospholipase C inhibitor, and RHC80267, which is a diacylglycerol lipase inhibitor, inhibited the effect of phenylephrine. These findings suggest that prostanoids, which are produced by constitutively active COX-2, influence the contractile response of the abdominal aorta and that the production of arachidonic acid relies on phospholipase C and diacylglycerol lipase. |
| Databáze: | OpenAIRE |
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