Susceptibility of neuroblastoma and glioblastoma cell lines to SARS-CoV-2 infection
| Autor: | Charles Nicaise, Kevin Willemart, Florian Poulain, Nicolas Gillet, Noémie Avalosse, Riselane Lotfi, Jacques Gilloteaux, Noelle Ninanne, Noémie Lejeune, Kathleen De Swert, Valery Bielarz |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cytoplasm viruses Cell Neuroblastoma 0302 clinical medicine RA retinoic acid tmprss2 transmembrane protease serine 2 BBB blood–brain barrier Cytopathic effect COVID-19 coronavirus disease 2019 ctsb cathepsin B General Neuroscience Serine Endopeptidases ish in situ hybridization medicine.anatomical_structure Neurotropism Angiotensin-Converting Enzyme 2 Cell type SARS-CoV severe acute respiratory syndrome coronavirus Clinical Neurology Biology Models Biological Article ctsl cathepsin L MOI multiplicity-of-infection 03 medical and health sciences Downregulation and upregulation Cell Line Tumor medicine Humans Molecular Biology Tropism hprt hypoxanthine–guanine phosphoribosyltransferase SARS-CoV-2 ace2 angiotensin-converting enzyme 2 COVID-19 medicine.disease Molecular biology 030104 developmental biology Cell culture Susceptibility Vero cell Neurology (clinical) Glioblastoma 030217 neurology & neurosurgery SARS-CoV-2 severe acute respiratory syndrome coronavirus-2 Developmental Biology |
| Zdroj: | Brain Research Bielarz, V, WILLEMART, KEVIN, Avalosse, N, De Swert, K, Lotfi, R, Lejeune, N, Poulain, F, Ninanne, N, GILLOTEAUX, J, GILLET, NICOLAS & Nicaise, C 2021, ' Susceptibility of neuroblastoma and glioblastoma cell lines to SARS-CoV-2 infection ', Brain research, vol. 1758, 147344 . https://doi.org/10.1016/j.brainres.2021.147344 |
| ISSN: | 1872-6240 |
| DOI: | 10.1016/j.brainres.2021.147344 |
| Popis: | Modelling cell infection in-a-dish can represent a useful tool to understand the susceptibility of different cell types towards severe acute respiratory coronavirus-2 (SARS-CoV-2) and to decipher its neurotropism. In this perspective, retinoic acid (RA)-differentiated neuroblastoma cell lines, SH-SY5Y and SK-N-BE(2) and glioblastoma cell lines, U-87 MG and U-373 MG, were infected with a SARS-CoV-2 strain, at various multiplicity-of-infection (MOI). We first demonstrated that the common entry genes – needed for invading epithelial cells – were expressed. RA-differentiation induced an upregulation of ace2 and tmprss2 gene expression while inducing downregulation of ctsb and ctsl. Using in situ hybridization and confocal analysis, SARS-CoV-2 gene S RNA was detected intracellularly at MOI 5.0, and localized in both soma and neuritic-like or glial-like processes. The infection was confirmed by quantification of viral gene E RNA and showed a dose-dependency, with few infected cells at MOI 0.1. After 24 h of infection, no cytopathic effect was observed in SH-SY5Y abilities to maintain neuritic processes or in U-373 MG for the uptake of glutamate. Unlike the permissive Vero E6 cells, no significant apoptosis death was detected following SARS-CoV-2 infection of neuroblastoma or glioblastoma cells. This study demonstrates the susceptibility of neuronal- and glial-like cell lines towards SARS-CoV-2 infection at high MOIs. Once inside the cells, the virus does not seem to rapidly replicate nor exert major cytopathic effect. Overall, our results strengthen the idea that SARS-CoV-2 has a tropism for nervous cells that express commonly described entry genes. |
| Databáze: | OpenAIRE |
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