Reactivity of bacterial and fungal laccases with lignin under alkaline conditions
| Autor: | Manuel Tenés Hernández, Maija-Liisa Mattinen, Anna Suurnäkki, Enriqueta Arias, Päivi Saastamoinen, Raquel Moya |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Acetosyringone
Environmental Engineering alkali lignin Bioengineering Streptomyces ipomoea Alkalies Redox Lignin Lignans Enzyme catalysis Polymerization Substrate Specificity chemistry.chemical_compound Oxygen Consumption Phenols Organic chemistry Reactivity (chemistry) Waste Management and Disposal Secoisolariciresinol Lignan Laccase Renewable Energy Sustainability and the Environment Fungi General Medicine Hydrogen-Ion Concentration Streptomyces Molecular Weight chemistry Biochemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Chromatography Gel Oxidation-Reduction Melanocarpus albomyces |
| Zdroj: | Moya, R, Saastamoinen, P, Hernández, M, Suurnäkki, A, Arias, E & Mattinen, M-L 2011, ' Reactivity of bacterial and fungal laccases with lignin under alkaline conditions ', Bioresource Technology, vol. 102, no. 21, pp. 10006-10012 . https://doi.org/10.1016/j.biortech.2011.08.046 |
| DOI: | 10.1016/j.biortech.2011.08.046 |
| Popis: | The ability of Streptomyces ipomoea laccase to polymerize secoisolariciresinol lignan and technical lignins was assessed. The reactivity of S. ipomoea laccase was also compared to that of low redox fungal laccase from Melanocarpus albomyces using low molecular mass p-coumaric, ferulic and sinapic acid as well as natural (acetosyringone) and synthetic 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO) mediators as substrates. Oxygen consumption measurement, MALDI-TOF MS and SEC were used to follow the enzymatic reactions at pH 7, 8, 9 and 10 at 30 °C and 50 °C. Polymerization of lignins and lignan by S. ipomoea laccase under alkaline reaction conditions was observed, and was enhanced in the presence of acetosyringone almost to the level obtained with M. albomyces laccase without mediator. Reactivities of the enzymes towards acetosyringone and TEMPO were similar, suggesting exploitation of the compounds and low redox laccase in lignin valorization under alkaline conditions. The results have scientific impact on basic research of laccases. |
| Databáze: | OpenAIRE |
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