Polysaccharide from Gracilaria caudata protects the human esophageal mucosal barrier: A differential topical effect and structural dependence
| Autor: | Ana Lúcia Ponte Freitas, Poliana de Oliveira Cavalcante Alencar, Renan O. Silva, Miguel Ângelo Nobre-Souza, Armênio Aguiar dos Santos, Lucas A.D. Nicolau, Francisco F. Bezerra, Rhubens Levy Moreira Rodrigues, Humberto Barbosa da Costa-Filho, Gustavo R.C. Santos, Thiago M. Sales, Pedro Marcos Gomes Soares, Francisco Clark Nogueira Barros, Marcellus H.L.P. Souza, Suliana Mesquita Paula, Renata Rocha Nascimento, Daniel Sifrim |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty Nerd Biopsy 02 engineering and technology Polysaccharide Protective Agents Biochemistry Gastroenterology 03 medical and health sciences Young Adult Sulfation Esophagus Structural Biology Polysaccharides Internal medicine medicine Gracilaria Humans Molecular Biology 030304 developmental biology Caudata Aged chemistry.chemical_classification 0303 health sciences Biological Products Mucous Membrane Ussing chamber Chemistry Hydrolysis Spectrum Analysis Reflux General Medicine Middle Aged 021001 nanoscience & nanotechnology medicine.disease In vitro GERD Gastroesophageal Reflux Female 0210 nano-technology |
| Zdroj: | International journal of biological macromolecules. 150 |
| ISSN: | 1879-0003 |
| Popis: | This study aimed to evaluate the in vitro protective effect of topical treatment with a native sulfated polysaccharide of G. caudata (SP-Gc), hydrolyzed (H-SP-Gc), or desulfated (D-SP-Gc) polysaccharide of Gracilaria caudata in esophageal biopsies obtained from GERD patients. Biopsies were obtained from nonerosive reflux disease (NERD) patients and from erosive esophagitis patients. Then, the biopsies were mounted in an Ussing chamber to measure the basal transepithelial electrical resistance (TEER). The effect of mucosal exposure to an acid solution on TEER was analyzed with or without different concentrations (1, 0.3 or 1%) of SP-Gc, H-SP-Gc, or D-SP-Gc, precoated on the mucosa. Basal esophageal mucosal electrical resistance was significantly lower in erosive esophagitis than from NERD. Mucosal samples precoated with native SP-Gc (1%) significantly prevented TEER drop induced by an acidic solution in NERD, but this effect was not observed in erosive esophagitis. Topical application of D-SP-Gc showed no difference compared to native SP-Gc. However, when treated with chemically-modified SP-Gc, the protective effect observed with native SP-Gc was lost. The present study indicated that SP-Gc protects the human esophageal mucosal barrier in NERD patients. This effect is dependent on the structure but is independent of the presence of sulfate. |
| Databáze: | OpenAIRE |
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