2267. The Effect of Opportunistic Infection (OI) Prophylaxis on the Gastrointestinal Microbiome (GIM) and Immune Reconstitution (IR) in Veterans With HIV and AIDS
| Autor: | Michelle M. Nerandzic, Curtis J. Donskey, Robert A. Bonomo, Jennifer L. Cadnum, Samira Joussef-Piña, Anne Mihelich-Ross, Marion J. Skalweit, Miguel E. Quiñones-Mateu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
business.industry
Opportunistic infection Gastrointestinal Microbiome Human immunodeficiency virus (HIV) medicine.disease medicine.disease_cause bacterial infections and mycoses urologic and male genital diseases female genital diseases and pregnancy complications Abstracts Infectious Diseases Immune system Oncology Acquired immunodeficiency syndrome (AIDS) B. Poster Abstracts Immunology Medicine business |
| Zdroj: | Open Forum Infectious Diseases |
| ISSN: | 2328-8957 |
| Popis: | Background Despite antiretroviral therapy (ART), some patients do not achieve IR. Moreover, GI bacterial translocation may lead to a decrease in CD4 counts with an increase in IL-6 in blood. However, the effect of OI prophylaxis on the GIM, bacterial translocation and IR has not been studied in HIV+ veterans. Here we studied the gut microbiome and bacterial translocation in VA patients with (i) stable HIV on ART (controls), (ii) newly diagnosed HIV starting on ART (new dx) and OI prophylaxis, and (iii) resuming ART (resumers). Methods Blood and stool specimens from 16 controls, 4 new dx and 3 resumers were obtained at 3 visits, as well as clinical and virological data. PCR electrospray ionization mass spectrometry (ESI-MS) was performed on blood samples to detect bacteria. Quantitative cultures and gut microbiome (deep sequencing bacterial 16S rRNA) was done on stool. Results There was no relation between CD4 count, log CFU TMP-SMX-resistant Gram-negative bacteria (GNRs) or total anaerobes. Except for 2 control patients with a decrease in CD4 count |
| Databáze: | OpenAIRE |
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