Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins
| Autor: | W. L. Muth, R. M. Van Frank, Daniel C. Williams, J. P. Burnett |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Multidisciplinary
biology Cytoplasmic inclusion DNA Recombinant medicine.disease_cause biology.organism_classification Cytoplasmic Granules Fusion protein Inclusion bodies law.invention Microscopy Electron Biochemistry law medicine Recombinant DNA Escherichia coli Humans Insulin Cloning Molecular Gene Bacteria Proinsulin Plasmids |
| Zdroj: | Science (New York, N.Y.). 215(4533) |
| ISSN: | 0036-8075 |
| Popis: | Escherichia coli that has been genetically manipulated by recombinant DNA technology to synthesize human insulin polypeptides (A chain, B chain, or proinsulin) contains prominent cytoplasmic inclusion bodies. The amount of inclusion product within the cells corresponds to the quantity of chimeric protein formed by the bacteria. At peak production, the inclusion bodies may occupy as much as 20 percent of the Escherichia coli cellular volume. |
| Databáze: | OpenAIRE |
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