Establishment and optimization of a hydroponic system for root morphological and nutritional analysis of citrus
| Autor: | Gaofeng Zhou, Qingjiang Wei, Bixian Li, Xiaoli Zeng, Guidong Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Agriculture (General) media_common.quotation_subject plant nutrition Biology 01 natural sciences Adaptability S1-972 Nutritional analysis root morphology trifoliate orange media_common Hydroponic culture food and beverages Plant physiology 04 agricultural and veterinary sciences Hydroponics biology.organism_classification Trifoliate orange Horticulture cultivation 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Aeration Plant nutrition 010606 plant biology & botany |
| Zdroj: | Scientia Agricola, Vol 77, Iss 4 (2019) Scientia Agricola v.77 n.4 2020 Scientia Agrícola Universidade de São Paulo (USP) instacron:USP Scientia Agricola, Volume: 77, Issue: 4, Article number: e20180261, Published: 04 NOV 2019 |
| ISSN: | 1678-992X |
| DOI: | 10.1590/1678-992x-2018-0261 |
| Popis: | The hydroponic growth system is a convenient platform for studying whole plant physiology, especially for root morphological and nutritional analysis. However, we found that most hydroponic systems described in the literature are not suitable for citrus plants. In this study, a hydroponic system for citrus was designed, comprising three principal components: power and time switch, aeration and hydroponic culture. Herein, details of the protocol were described, including equipment setup, seed pregermination and cultivation, together with preparation and transfer of nutrient solution into hydroponics. In order to demonstrate the adaptability of the trifoliate orange plant to our hydroponic system, comparative tests between soil- and hydroponically-grown plants were carried out. The results showed that the plants grew normally and there were no obvious differences between soil- and hydroponically-grown plants. In addition, nutrient deficiency and transcriptional analysis were carried out to test the efficiency, functionality and suitability of our hydroponic system for the application of physiological and molecular analysis. The results, compared with previous studies, showed that our hydroponic system delivered superior performance as regards the physiological and molecular analysis. Taken together, we established the culture system which is best suited for the growth of trifoliate oranges under hydroponic conditions. The hydroponic system described in this paper is easily constructed and controlled at a low cost. It may serve a wide gamut of experimental purposes, especially root morphological and nutritional analysis of trifoliate oranges and the system is also adaptable to other citrus plants by varying the device size. |
| Databáze: | OpenAIRE |
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