Genetic Identification and Characterization of Limestone Canyon Virus, a Unique Peromyscus-Borne Hantavirus
| Autor: | Ken D. Abbott, Stuart T. Nichol, Angela J. Sanchez |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Orthohantavirus
Peromyscus RNA virus Hantavirus Infections animal diseases viruses Molecular Sequence Data Reithrodontomys Biology Antibodies Viral Polymerase Chain Reaction Virus hantavirus Rodent Diseases Peromyscus boylii Virology medicine Animals Limestone Canyon virus genetics Phylogeny Hantavirus Hantavirus pulmonary syndrome Reverse Transcriptase Polymerase Chain Reaction Sin Nombre virus virus diseases Sequence Analysis DNA medicine.disease biology.organism_classification DNA Viral coevolution RNA Viral |
| Zdroj: | Virology. 286(2):345-353 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.2001.0983 |
| Popis: | Hantaviruses, family Bunyaviridae, are rodent-borne RNA viruses that can cause hantavirus pulmonary syndrome (HPS) in various regions of the Americas. A coevolutionary relationship exists between hantaviruses and their specific rodent reservoir hosts; the phylogeny of the viruses generally matches that of the rodents. There are several Peromyscus -borne hantaviruses, including Sin Nombre virus, the most common cause of HPS in North America. This report describes the genetic detection and characterization of a newly discovered Peromyscus boylii -borne virus, Limestone Canyon (LSC) virus, the most divergent member of the Peromyscus -borne hantaviruses to date. Analysis of a 1209-nucleotide region of the S segment of LSC virus showed it to be more closely related to hantaviruses found in harvest mice ( Reithrodontomys megalotis and R. mexicanus ) than to other Peromyscus -associated hantaviruses (Sin Nombre, New York, and Monongahela). Phylogenetic analysis of virtually the entire M genome segment (3489 nucleotides) of LSC virus revealed a similar picture in which LSC virus was found to be very distinct from other Peromyscus -associated viruses, but its exact relationship to the other Peromyscus -borne and the Reithrodontomys -borne viruses was not resolved. These results indicate that hantavirus host species-jumping events can occur by which a hantavirus may switch to, and become established in, a rodent host belonging to a different genus. P. boylii are present throughout the southwestern United States and central Mexico. More extensive screening of HPS patients by using RT–PCR assays will be necessary to determine if LSC virus can cause human disease. |
| Databáze: | OpenAIRE |
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