Immunoassay Multiplexing on a Complementary Metal Oxide Semiconductor Photodiode Array
Autor: | Boon Chong Cheah, David R. S. Cumming, Bence Nagy, Michael P. Barrett, Mohammed A. Al-Rawhani |
---|---|
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Materials science Metal Nanoparticles Bioengineering Biosensing Techniques Integrated circuit HIV Envelope Protein gp120 01 natural sciences Multiplexing Antibodies Article law.invention Mice 03 medical and health sciences Sensor array Limit of Detection law diagnostics medicine Animals Humans Multiplex Instrumentation Immunoassay Fluid Flow and Transfer Processes CMOS sensor medicine.diagnostic_test business.industry Process Chemistry and Technology CMOS 010401 analytical chemistry HIV Oxides Mobile Applications 0104 chemical sciences Photodiode 030104 developmental biology Semiconductors silver enhancement Metals point-of-care Optoelectronics Gold Rabbits business |
Zdroj: | ACS Sensors |
ISSN: | 2379-3694 |
DOI: | 10.1021/acssensors.7b00972 |
Popis: | Scalable immunoassay multiplexing offers a route to creating rapid point-of-care (POC) diagnostics. We present a method for multiplexing immunoassays on the surface of a complementary metal oxide semiconductor (CMOS) sensor array integrated circuit (IC) without the use of physical separators such as wells or channels. Major advantages of using a CMOS sensor array include low mass-manufacturing costs, the possibility to multiplex multiple assays on a single IC, and improved signal when averaging multiple sensors, along with providing a platform where wash steps can be incorporated to maximize selectivity and sensitivity compared to paper based lateral flow immunoassay. The device was able to differentiate between samples containing either, neither, or both rabbit anti-mouse (RAM) antibodies and/or anti-HIV gp120 antibodies in serum using a gold-nanoparticle promoted silver enhancement immunoassay. HIV antibody concentrations down to 100 μg/mL were readily detected, which is three times lower than those typically found in infected humans (300–500 μg/mL), and the limit of detection was 10 μg/mL. |
Databáze: | OpenAIRE |
Externí odkaz: |