Identification of 4-n-nonylphenol metabolic pathways and residues in aquatic organisms by HPLC and LC-MS analyses
| Autor: | E. Rathahao, L. Lagadic, J.-P. Cravedi, Laurent Debrauwer, R. Thibaut, A. Jumel |
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| Přispěvatelé: | Xénobiotiques, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2000 |
| Předmět: |
risque biologique
endocrine system [SDV]Life Sciences [q-bio] lymnaea xénobiotique composé phénolique Lymnaea stagnalis hplc 010501 environmental sciences 01 natural sciences lemna chemistry.chemical_compound poisson mollusque SALMONIDAE ONCORHYNCHUS MYKISS pollution [INFO]Computer Science [cs] gastéropode ComputingMilieux_MISCELLANEOUS Salmonidae 0105 earth and related environmental sciences biology urogenital system 010401 analytical chemistry General Engineering General Chemistry biology.organism_classification 0104 chemical sciences Nonylphenol Trout Metabolic pathway bioaccumulation poisson osseux chemistry Endocrine disruptor Biochemistry lymnaea stagnalis lentille d'eau technique analytique Rainbow trout Xenobiotic truite arc en ciel |
| Zdroj: | Analusis Analusis, EDP Sciences, 2000, 28 (9), pp.793-801 Analusis 9 (28), 793-801. (2000) |
| ISSN: | 1286-482X 0365-4877 |
| DOI: | 10.1051/analusis:2000280793 |
| Popis: | In order to better assess the risk associated with nonylphenol, a lipophilic contaminant of the aquatic environment having a strong tendency to bioaccumulate in organisms and exerting estrogenic effects in fish and invertebrates, we have investigated the capability of three types of freshwater species to metabolize labelled 4-n-nonylphenol. Reverse-phase radio-HPLC was used to isolate and quantify the metabolites. The identification of residues was based on co-chromatography with available standards or authenfified metabolites and when possible by electrospray ionization LC-MS analyses. The residues found in rainbow trout (Oncorhynchus mykiss), pond snails (Lymnea stagnalis) and duckweeds (Lemna minor) were identified. These freshwater organisms are able to extensively biotransform 4-n-nonylphenol. Our attempts to draw the metabolic pathways of nonlyphenol evidenced oxidative attack on the alkyl chain with the production of several hydroxylated compounds and related carboxylic acids. In addition, trout and duck-weeds were able to conjugate the phenol moiety to glucuronic acid and carbohydrates respectively. Traces of sulphate conjugates were also detected in trout tissues whereas no conjugate was detected in pond snails. |
| Databáze: | OpenAIRE |
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