Altered sialylation on the cell-surface proteins of dexamethasone-treated human macrophages contributes to augmented uptake of apoptotic neutrophils
| Autor: | Attila Szanto, György Vámosi, András Mádi, Michael O. Glocker, Gyöngyike Majai, Cornelia Koy, László Fésüs |
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| Rok vydání: | 2011 |
| Předmět: |
Programmed cell death
Necrosis Neutrophils Phagocytosis Immunology Anti-Inflammatory Agents Ribosome Inactivating Proteins Apoptosis Inflammation Dexamethasone medicine Humans Immunology and Allergy Elméleti orvostudományok biology Macrophages Membrane Proteins Lectin Orvostudományok N-Acetylneuraminic Acid Cell biology Biochemistry biology.protein Plant Lectins medicine.symptom Glucocorticoid medicine.drug |
| Zdroj: | Immunology Letters. 135:88-95 |
| ISSN: | 0165-2478 |
| Popis: | Macrophages eliminate apoptotic granulocytes before their secondary necrosis during resolution of inflammation. A well-known glucocorticoid, the anti-inflammatory dexamethasone augments phagocytosis capacity of macrophages with a so far not fully clarified mechanism. We have found that sialylation of cell-surface proteins on human macrophages is markedly altered by dexamethasone. Compared to non-treated cells, dexamethasone-treated macrophages can bind significantly less Sambucus nigra lectin specific for sialic acids on their surfaces as a result of undersialylation of annexin-II and an HLA-II protein. Non-treated macrophages covered by S. nigra lectin had increased uptake of apoptotic cells; however, the significantly higher phagocytosis capacity of dexamethasone-treated macrophages could not be stimulated further this way. Our results suggest that dexamethasone treatment leads to decreased number of sialic acids on the surfaces of human macrophages promoting recognition and uptake of apoptotic cells. |
| Databáze: | OpenAIRE |
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