Actin impacts the late stages of prion formation and prion propagation
| Autor: | Brett T. Wisniewski, Jane E. Dorweiler, Legan Er, Davis Ee, Anita L. Manogaran, Douglas R. Lyke |
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| Rok vydání: | 2017 |
| Předmět: |
0303 health sciences
Cell division biology animal diseases 030302 biochemistry & molecular biology Mutant Actin remodeling Arp2/3 complex macromolecular substances Yeast nervous system diseases Cell biology 03 medical and health sciences Fimbrin biology.protein MDia1 Actin 030304 developmental biology |
| Popis: | In yeast, the [PSI+] and [PIN+] prions are aggregated forms of the Sup35 and Rnq1 proteins, respectively. The cellular mechanisms that underlie the formation and propagation of these prion states are not clearly understood. Our previous work suggested that actin networks play a role in early and late steps of the formation of [PSI+]. To further explore how actin impacts yeast prions, we turned to a set of actin point mutants. We found that the disruption of actin cables, either by an actin destabilizing drug or theact1-101mutant, can enhance prion formation during the later stages of prion formation. Our data suggest that under normal conditions, actin cables play a role in limiting the inheritance of newly made prion particles to daughter cells. We also found actin can impact prion propagation. Theact1-122mutant, which contains a substitution in the fimbrin binding region, destabilized the [PIN+] prion over time. This is the first evidence that actin has a role in [PIN+] propagation. Taken together, our findings reveal novel roles for actin in the formation and propagation of prions. |
| Databáze: | OpenAIRE |
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