Continuously Grooved Stent Struts for Enhanced Endothelial Cell Seeding

Autor: Willeke F. Daamen, J. Adam van der Vliet, Jeremy E. Schaffer, Lambertus P. van den Heuvel, Gijs van Son, Marja ter Meer, Leo J. Schultze Kool, Yvonne L. Hoogeveen
Rok vydání: 2017
Předmět:
medicine.medical_treatment
Vascular damage Radboud Institute for Health Sciences [Radboudumc 16]
Self Expandable Metallic Stents
Thrombogenicity
030204 cardiovascular system & hematology
In Vitro Techniques
030218 nuclear medicine & medical imaging
03 medical and health sciences
0302 clinical medicine
Restenosis
In vitro
Self-expandable metallic stent
Stent deployment
316L stainless steel
Alloys
Medicine
Humans
Radiology
Nuclear Medicine and imaging
Endovascular stent
Cell Proliferation
Endothelialization
business.industry
Other Research Radboud Institute for Health Sciences [Radboudumc 0]
Models
Cardiovascular
Stent
Cell adhesion
Endothelial Cells
Equipment Design
Nitinol
medicine.disease
equipment and supplies
Endothelial stem cell
Reconstructive and regenerative medicine Radboud Institute for Health Sciences [Radboudumc 10]
Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10]
Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11]
Pulsatile Flow
Laboratory Investigation
Feasibility Studies
Gelatin
Seeding
Cardiology and Cardiovascular Medicine
business
Biomedical engineering
Rare cancers Radboud Institute for Health Sciences [Radboudumc 9]
Zdroj: Cardiovascular and Interventional Radiology, 40, 1237-1245
Cardiovascular and Interventional Radiology
Cardiovascular and Interventional Radiology, 40, 8, pp. 1237-1245
ISSN: 0174-1551
Popis: Purpose Implantation of pre-endothelialized stents could enhance cellular recovery of a damaged vessel wall provided attached cells remain viable, functional and are present in sufficient numbers after deployment. The purpose of this study was to evaluate the feasibility of grooved stainless steel (SS) stents as a primary endothelial cell (EC) carrier with potentially enhanced EC protection upon stent deployment. Materials and Methods Attachment and behavior of enzymatically harvested human adult venous ECs seeded onto gelatin-coated vascular stents were evaluated in an in vitro setting. Smooth and grooved SS stents and smooth nitinol stents were studied. Results All cells expressed EC markers vWF and CD31. Using rotational seeding for a period of 16–24 h, ECs attached firmly to the stents with sufficient coverage to form a confluent EC monolayer. The grooved SS wire design was found to enable attachment of three times the number of cells compared to smooth wires. This also resulted in an increased number of cells remaining on the stent after deployment and after pulsatile flow of 180 ml/min for 24 h, which did not result in additional EC detachment. Conclusions The grooved stent provides a potential percutaneous means to deliver sufficient numbers of viable and functional cells to a vessel segment during vascular intervention. The grooves were found to offer a favorable surface for EC attachment and protection during stent deployment in an in vitro setting. Electronic supplementary material The online version of this article (doi:10.1007/s00270-017-1659-4) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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