Bone Marrow-Derived Mononuclear Cells Promote Improvement in Glomerular Function in Rats with Early Diabetic Nephropathy
| Autor: | Bernardo Miguel de Oliveira Pascarelli, Bruno L. Diaz, Patricia R. M. Rocco, Carolina M.L. Barbosa, Tatiana Maron-Gutierrez, Jackson Souza-Menezes, Felipe M. Ornellas, Christina Maeda Takiya, Raquel C. Castiglione, Carolina B A Dibarros, Bruno Diaz Paredes, André Luis Barreira, Marcelo M. Morales, Bartira Rossi-Bergmann, Andréia Vasconcelos-dos-Santos |
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| Rok vydání: | 2013 |
| Předmět: |
Blood Glucose
Male Vascular Endothelial Growth Factor A medicine.medical_specialty Arginase I+ macrophages Physiology Renal function Bone Marrow Cells Diabetic nephropathy Kidney Peripheral blood mononuclear cell lcsh:Physiology Diabetes Mellitus Experimental Transforming Growth Factor beta1 lcsh:Biochemistry Excretion Internal medicine Diabetes mellitus medicine Animals Diabetic Nephropathies lcsh:QD415-436 Rats Wistar Proteinuria Arginase lcsh:QP1-981 Interleukin-6 business.industry Macrophages Body Weight Ectodysplasins medicine.disease Streptozotocin Interleukin-10 Rats Endocrinology Leukocytes Mononuclear Bone marrow mononuclear cells Cytokines medicine.symptom business Glomerular Filtration Rate medicine.drug |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 32, Iss 3, Pp 699-718 (2013) |
| ISSN: | 1421-9778 1015-8987 |
| DOI: | 10.1159/000354473 |
| Popis: | Background/Aims: Diabetic nephropathy is one of the main causes of end-stage renal disease. The present study investigated the effect of mononuclear cell (MC) therapy in rats subjected to diabetic nephropathy. Methods: Male Wistar rats were divided into control (CTRL), diabetic (DM), CTRL+MC and DM+MC groups. Diabetes was induced by a single injection of streptozotocin (45 mg/kg, i.p.) and, 4 weeks later, 2×107 MCs were injected via the jugular vein. Results: The rats in the DM and DM+MC groups showed increased glycemia, glomerular filtration rate and glomerular tuff area versus control groups. The glomerular filtration rate and glomerular tuff area were normalized in the DM+MC group. No alterations were observed in the fractional excretion of electrolytes and proteinuria between the DM and DM+MC groups. TGF-β1 protein levels in the DM group were significantly increased versus control animals and normalized in the DM+MC group. An increase in ED1+/arginase I+ macrophages and IL-10 renal expression was observed in the DM+MC group versus DM group. Conclusions: Bone marrow-derived MC therapy was able to prevent glomerular alterations and TGF-β1 protein overexpression and modulated glomerular arginase I+ macrophage infiltration in rats subjected to early diabetic nephropathy. |
| Databáze: | OpenAIRE |
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