Dose-dependent effects of gonadotropin on oocyte developmental competence and apoptosis
| Autor: | Shan Liu, A. Hershlag, Zi-Jiang Chen, Huai L. Feng, Dennis Marchesi |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
endocrine system medicine.drug_class Embryonic Development Apoptosis Fertilization in Vitro Reproductive technology Biology Oogenesis Tissue Culture Techniques Andrology Endocrinology Genetics medicine Animals Blastocyst Molecular Biology Cell Nucleus Cumulus Cells Dose-Response Relationship Drug Embryo culture Anatomy Embryo Mammalian Oocyte In vitro maturation medicine.anatomical_structure Reproductive Medicine Oocytes Cattle Female Animal Science and Zoology Folliculogenesis Gonadotropin Gonadotropins Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 23:990 |
| ISSN: | 1031-3613 |
| Popis: | The aim of the present study was to evaluate the effect of gonadotropins (Gn) on oocyte maturation, developmental competence and apoptosis in an animal model. Bovine cumulus–oocyte complexes (COCs) were matured for 24 h in media supplemented with varying concentrations of Bravelle (B), B + Menopur (B + M) or B + Repronex (B + R) (Ferring Pharmaceuticals, Parsiappany, NJ, USA). Then, nuclear maturation, embryo development, and apoptosis in cumulus cells and oocytes were evaluated. Low to moderate Gn concentrations (75–7500 mIU mL–1) effectively improved nuclear maturation and in vitro development. Higher concentrations of Gn (75 000 mIU mL–1) did not have any added beneficial effects and nuclear maturation and blastocyst rates in the presence of these concentrations were comparable to control (P > 0.05). Most COCs showed slight apoptosis when exposed to 75, 750 and 7500 mIU mL–1 Gn; however, when the concentration was increased to 75 000 mIU mL–1, the proportion of moderately apoptotic COCs increased. In conclusion, extremely high concentrations of Gn have detrimental effects on oocyte nuclear maturation and embryo development and increase apoptosis in cumulus cells, suggesting the importance of judicious use of Gn in assisted reproductive technologies (ART). |
| Databáze: | OpenAIRE |
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